LSBio
Human STXBP5 / Tomosyn Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human STXBP5 / Tomosyn Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G93528-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Tomosyn(STXBP5) (NM_001127715) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LGL3 | STXBP5 | Tomosyn | Nbla04300 | Syntaxin-binding protein 5 | LLGL3 | Tomosyn-1
Origin Species
Human
SwissProt
Q5T5C0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
127.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRKFNIRKVL DGLTAGSSSA SQQQQQQHPP GNREPEIQET LQSEHFQLCK TVRHGFPYQP
61SALAFDPVQK ILAVGTQTGA LRLFGRPGVE CYCQHDSGAA VIQLQFLINE GALVSALADD
121TLHLWNLRQK RPAILHSLKF CRERVTFCHL PFQSKWLYVG TERGNIHIVN VESFTLSGYV
181IMWNKAIELS SKSHPGPVVH ISDNPMDEGK LLIGFESGTV VLWDLKSKKA DYRYTYDEAI
241HSVAWHHEGK QFICSHSDGT LTIWNVRSPA KPVQTITPHG KQLKDGKKPE PCKPILKVEF
301KTTRSGEPFI ILSGGLSYDT VGRRPCLTVM HGKSTAVLEM DYSIVDFLTL CETPYPNDFQ
361EPYAVVVLLE KDLVLIDLAQ NGYPIFENPY PLSIHESPVT CCEYFADCPV DLIPALYSVG
421ARQKRQGYSK KEWPINGGNW GLGAQSYPEI IITGHADGSV KFWDASAITL QVLYKLKTSK
481VFEKSRNKDD RPNTDIVDED PYAIQIISWC PESRMLCIAG VSAHVIIYRF SKQEVITEVI
541PMLEVRLLYE INDVETPEGE QPPPLPTPVG GSNPQPIPPQ SHPSTSSSSS DGLRDNVPCL
601KVKNSPLKQS PGYQTELVIQ LVWVGGEPPQ QITSLAVNSS YGLVVFGNCN GIAMVDYLQK
661AVLLNLGTIE LYGSNDPYRR EPRSPRKSRQ PSGAGLCDIS EGTVVPEDRC KSPTSGSSSP
721HNSDDEQKMN NFIEKVKTKS RKFSKMVAND IAKMSRKLSL PTDLKPDLDV KDNSFSRSRS
781SSVTSIDKES REAISALHFC ETFTRKTDSS PSPCLWVGTT LGTVLVIALN LPPGGEQRLL
841QPVIVSPSGT ILRLKGAILR MAFLDTTGCL IPPAYEPWRE HNVPEEKDEK EKLKKRRPVS
901VSPSSSQEIS ENQYAVICSE KQAKVISLPT QNCAYKQNIT ETSFVLRGDI VALSNSICLA
961CFCANGHIMT FSLPSLRPLL DVYYLPLTNM RIARTFCFTN NGQALYLVSP TEIQRLTYSQ
1021ETCENLQEML GELFTPVETP EAPNRGFFKG LFGGGAQSLD REELFGESSS GKASRSLAQH
1081IPGPGGIEGV KGAASGVVGE LARARLALDE RGQKLGDLEE RTAAMLSSAE SFSKHAHEIM
1141LKYKDKKWYQ F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1151 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.