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Human SSFA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SSFA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SSFA2 antibodies →
$153.00each
Catalog Number: LS-G75197-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SSFA2 (NM_006751) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cleavage signal-1 protein | CS1 | CS-1 | KIAA1927 | SPAG13 | SSFA2 | Sperm associated antigen 13 | Sperm specific antigen 2 | KRAP | Sperm-specific antigen 2
Origin Species
Human
NCBI GenBank
NM_006751
SwissProt
P28290

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
137.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRPLSSSAE AEEELEWQVA SRRRKAWAKC RSSWQASETE DLSTEATTQD EEEDEEEDLP
61GAQLPAAGGR GNVPNEKIAI WLKDCRTPLG ASLDEQSSST LKGVLVRNGG SFEDDLSLGA
121EANHLHESDA QIENCNNILA KERRLQFHQK GRSMNSTGSG KSSGTVSSVS ELLELYEEDP
181EEILYNLGFG RDEPDIASKI PSRFFNSSSF AKGIDIKVFL SAQMQRMEVE NPNYALTSRF
241RQIEVLTTVA NAFSSLYSQV SGTPLQRIGS MSSVTSNKET DPPPPLTRSN TANRLMKTLS
301KLNLCVDKTE KGESSSPSPS AEKGKILNVS VIEESGNKND QKSQKIMKKK ESSSMLATVK
361EEVSGSSAAV TENADSDRIS DEANSNFNQG TENEQSKETQ SHESKLGEES GIVESKLDSD
421FNISSHSELE NSSELKSVHI STPEKEPCAP LTIPSIRNIM TQQKDSFEME EVQSTEGEAP
481HVPATYQLGL TKSKRDHLLR TASQHSDSSG FAEDSTDCLS LNHLQVQESL QAMGSSADSC
541DSETTVTSLG EDLATPTAQD QPYFNESEEE SLVPLQKGLE KAAAVADKRK SGSQDFPQCN
601TIENTGTKQS TCSPGDHIIE ITEVEEDLFP AETVELLREA SAESDVGKSS ESEFTQYTTH
661HILKSLASIE AKCSDMSSEN TTGPPSSMDR VNTALQRAQM KVCSLSNQRM GRSLLKSKDL
721LKQRYLFAKA GYPLRRSQSL PTTLLSPVRV VSSVNVRLSP GKETRCSPPS FTYKYTPEEE
781QELEKRVMEH DGQSLVKSTI FISPSSVKKE EAPQSEAPRV EECHHGRTPT CSRLAPPPMS
841QSTCSLHSIH SEWQERPLCE HTRTLSTHSV PNISGATCSA FASPFGCPYS HRHATYPYRV
901CSVNPPSAIE MQLRRVLHDI RNSLQNLSQY PMMRGPDPAA APYSTQKSSV LPLYENTFQE
961LQVMRRSLNL FRTQMMDLEL AMLRQQTMVY HHMTEEERFE VDQLQGLRNS VRMELQDLEL
1021QLEERLLGLE EQLRAVRMPS PFRSSALMGM CGSRSADNLS CPSPLNVMEP VTELMQEQSY
1081LKSELGLGLG EMGFEIPPGE SSESVFSQAT SESSSVCSGP SHANRRTGVP STASVGKSKT
1141PLVARKKVFR ASVALTPTAP SRTGSVQTPP DLESSEEVDA AEGAPEVVGP KSEVEEGHGK
1201LPSMPAAEEM HKNVEQDELQ QVIREVGMDP ISCVILELSM ICTGGGVICA LEDTCC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1256 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.