LSBio
Human SPR Protein (Recombinant His)

Human SPR Protein (Recombinant His)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
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$267.00each
Catalog Number: LS-G509-20

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Overview

Description
Sepiapterin reductase (SPR) belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. SPR is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in SPR cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant human SPR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
SPR | SDR38C1 | Sepiapterin reductase
Origin Species
Human
NCBI GenBank
NM_003124|NP_003115.1
SwissProt
P35270

Protein

Expression System
Escherichia coli (Prokaryotic)
Fusion Tag
Hexahistidine Tag
Protein Type
Recombinant
Amino Acid Sequence
11-261aa|MG SSHHHHHHSS GLVPRGSHME GGLGRAVCLL TGASRGFGRT LAPLLASLLS
61PGSVLVLSAR NDEALRQLEA ELGAERSGLR VVRVPADLGA EAGLQQLLGA LRELPRPKGL
121QRLLLINNAG SLGDVSKGFV DLSDSTQVNN YWALNLTSML CLTSSVLKAF PDSPGLNRTV
181VNISSLCALQ PFKGWALYCA GKAARDMLFQ VLALEEPNVR VLNYAPGPLD TDMQQLARET
241SVDPDMRKGL QELKAKGKLV DCKVSAQKLL SLLEKDEFKS GAHVDFYDK

289 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Liquid
Formulation
20 mM Tris-HCl, pH 8.0, 10% Glycerol
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.

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