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Human SPOCD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPOCD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPOCD1 antibodies →
$220.00each
Catalog Number: LS-G96317-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPOCD1 (NM_144569) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_144569

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQAGDVEGP STGDPVLSPQ HNCELLQNME GASSMPGLSP DGPGASSGPG VRAGSRRKIP
61RKEALRGGSS RAAGAAEVRP GVLELLAVVQ SRGSMLAPGL HMQLPSVPTQ GRALTSKRLQ
121VSLCDILDDS CPRKLCSRSA GLPERALACR ERLAGVEEVS CLRPREARDG GMSSPGCDRR
181SPTLSKEEPP GRPLTSSPDP VPVRVRKKWR RQGAHSECEE GAGDFLWLDQ SPRGDNLLSV
241GDPPQVADLE SLGGPCRPPS PKDTGSGPGE PGGSGAGCAS GTEKFGYLPA TGDGPQPGSP
301CGPVGFPVPS GGESLSSAAQ APPQSAALCL GASAQASAEQ QEAVCVVRTG SDEGQAPAQD
361QEELEAKAQP ASRGRLEQGL AAPADTCASS REPLGGLSSS LDTEASRACS GPFMEQRRSK
421GTKNLKKGPV PCAQDRGTDR SSDNSHQDRP EEPSPGGCPR LEEVKIPHGV KLVCYLGSGP
481VIQLLGAISH GQAGGQLPPK LEVLEDLMEV SSPSPAQRLR RKKRPMVQGP AGCQVFQPSP
541SGGTAGDPGG LSDPFYPPRS GSLALGDPSS DPACSQSGPM EAEEDSLPEQ PEDSAQLQQE
601KPSLYIGVRG TVVRSMQEVL WTRLRELPDP VLSEEVVEGI AAGIEAALWD LTQGTNGRYK
661TKYRSLLFNL RDPRNLDLFL KVVHGDVTPY DLVRMSSMQL APQELARWRD QEEKRGLNII
721EQQQKEPCRL PASKMTHKGE VEIQRDMDQT LTLEDLVGPQ MFMDCSPQAL PIASEDTTGQ
781HDHHFLDPNC HICKDWEPSN ELLGSFEAAK SCGDNIFQKA LSQTPMPAPE MPKTRELSPT
841EPQDRVPPSG LHVPAAPTKA LPCLPPWEGV LDMFSIKRFR ARAQLVSGHS CRLVQALPTV
901IRSAGCIPSN IVWDLLASIC PAKAKDVCVV RLCPHGARDT QNCRLLYSYL NDRQRHGLAS
961VEHMGMVLLP LPAFQPLPTR LRPLGGPGLW ALPVSPLLSP GLEVTHSSLL LAVLLPKEGL
1021PDTAGSSPWL GKVQKMVSFN SKVEKRYYQP DDRRPNVPLK GTPPPGGAWQ QSQGRGSIAP
1081RGISAWQRPP RGRGRLWPEP ENWQHPGRGQ WPPEPGLRQS QHPYSVAPAG HGFGRGQHFH
1141RDSCPHQALL RHLESLATMS HQLQALLCPQ TKSSIPRPLQ RLSSALAAPE PPGPARDSSL
1201GPTDEAGSEC PFPRKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1216 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.