LSBio
Human SPINT1 / HAI-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPINT1 / HAI-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPINT1 antibodies →
$220.00each
Catalog Number: LS-G79690-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPINT1 (NM_181642) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HAI1 | MANSC2 | HAI | SPINT1 | HAI-1
Origin Species
Human
NCBI GenBank
NM_003710
SwissProt
O43278

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPARTMARA RLAPAGIPAV ALWLLCTLGL QGTQAGPPPA PPGLPAGADC LNSFTAGVPG
61FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA LVELQPDRGE DAIAACFLIN
121CLYEQNFVCK FAPREGFINY LTREVYRSYR QLRTQGFGGS GIPKAWAGID LKVQPQEPLV
181LKDVENTDWR LLRGDTDVRV ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV
241LSTKQTEDYC LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC
301RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS FLECDDTPNC
361PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC KESIPRWYYN PFSEHCARFT
421YGGCYGNKNN FEEEQQCLES CRGISKKDVF GLRREIPIPS TGSVEMAVAV FLVICIVVVV
481AILGYCFFKN QRKDFHGHHH HPPPTPASST VSTTEDTEHL VYNHTTRPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

529 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.