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Human SPAG16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPAG16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPAG16 antibodies →
$220.00each
Catalog Number: LS-G82220-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPAG16 (NM_024532) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Pf20 protein homolog | WDR29 | PF20 | SPAG16 | Sperm associated antigen 16 | WD repeat domain 29
Origin Species
Human
NCBI GenBank
NM_024532
SwissProt
Q8N0X2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAQRGMPSS AVRVLEEALG MGLTAAGDAR DTADAVAAEG AYYLEQVTIT EASEDDYEYE
61EIPDDNFSIP EGEEDLAKAI QMAQEQATDT EILERKTVLP SKHAVPEVIE DFLCNFLIKM
121GMTRTLDCFQ SEWYELIQKG VTELRTVGNV PDVYTQIMLL ENENKNLKKD LKHYKQAADK
181AREDLLKIQK ERDFHRMHHK RIVQEKNKLI NDLKGLKLHY ASYEPTIRVL HEKHHTLLKE
241KMLTSLERDK VVGQISGLQE TLKKLQRGHS YHGPQIKVDH SREKENAPEG PTQKGLREAR
301EQNKCKTKMK GNTKDSEFPI DMQPNPNLNV SKESLSPAKF DYKLKNIFRL HELPVSCVSM
361QPHKDILVSC GEDRLWKVLG LPKCNVLLTG FGHTDWLSDC CFHPSGDKLA TSSGDTTVKL
421WDLCKGDCIL TFEGHSRAVW SCTWHSCGNF VASSSLDKTS KIWDVNSERC RCTLYGHTDS
481VNSIEFFPFS NTLLTSSADK TLSIWDARTG ICEQSLYGHM HSINDAIFDP RGHMIASCDA
541CGVTKLWDFR KLLPIVSIDI GPSPGNEVNF DSSGRVLAQA SGNGVIHLLD LKSGEIHKLM
601GHENEAHTVV FSHDGEILFS GGSDGTVRTW S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

631 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.