SP5 Antibody
Catalog Number: LS-C490120-100
100 µg / $587.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G95638-20
Catalog Number: LS-G95638
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SP5 (NM_001003845) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001003845
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAVAVLRND SLQAFLQDRT PSASPDLGKH SPLALLAATC SRIGQPGAAA PPDFLQVPYD61PALGSPSRLF HPWTADMPAH SPGALPPPHP SLGLTPQKTH LQPSFGAAHE LPLTPPADPS121YPYEFSPVKM LPSSMAALPA SCAPAYVPYA AQAALPPGYS NLLPPPPPPP PPPTCRQLSP181NPAPDDLPWW SIPQAGAGPG ASGVPGSGLS GACAGAPHAP RFPASAAAAA AAAAALQRGL241VLGPSDFAQY QSQIAALLQT KAPLAATARR CRRCRCPNCQ AAGGAPEAEP GKKKQHVCHV301PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKSFTRS DELQRHLRTH TGEKRFACPE361CGKRFMRSDH LAKHVKTHQN KKLKVAEAGV KREDARDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
398 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
