LSBio
Human SNTB1 / A1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SNTB1 / A1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G74781-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Syntrophin(SNTB1) (NM_021021) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
59-DAP | A1B | Beta-1-syntrophin | Beta1-syntrophin | BSYN2 | DAPA1B | SNT2B1 | Tax interaction protein 43 | SNT2 | SNTB1 | Syntrophin-2 | TIP-43
Origin Species
Human
NCBI GenBank
NM_021021
SwissProt
Q13884

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MAVAAAAAAA GPAGAGGGRA QRSGLLEVLV RDRWHKVLVN LSEDALVLSS EEGAAAYNGI
61GTATNGSFCR GAGAGHPGAG GAQPPDSPAG VRTAFTDLPE QVPESISNQK RGVKVLKQEL
121GGLGISIKGG KENKMPILIS KIFKGLAADQ TQALYVGDAI LSVNGADLRD ATHDEAVQAL
181KRAGKEVLLE VKYMREATPY VKKGSPVSEI GWETPPPESP RLGGSTSDPP SSQSFSFHRD
241RKSIPLKMCY VTRSMALADP ENRQLEIHSP DAKHTVILRS KDSATAQAWF SAIHSNVNDL
301LTRVIAEVRE QLGKTGIAGS REIRHLGWLA EKVPGESKKQ WKPALVVLTE KDLLIYDSMP
361RRKEAWFSPV HTYPLLATRL VHSGPGKGSP QAGVDLSFAT RTGTRQGIET HLFRAETSRD
421LSHWTRSIVQ GCHNSAELIA EISTACTYKN QECRLTIHYE NGFSITTEPQ EGAFPKTIIQ
481SPYEKLKMSS DDGIRMLYLD FGGKDGEIQL DLHSCPKPIV FIIHSFLSAK ITRLGLVA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

538 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.