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Human Smoothelin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human Smoothelin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SMTN antibodies →
$220.00each
Catalog Number: LS-G78863-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Smoothelin(SMTN) (NM_134270) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SMTN | Smoothelin | SMSMO
Origin Species
Human
NCBI GenBank
NM_006932
SwissProt
P53814

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADEALAGLD EGALRKLLEV TADLAERRRI RSAIRELQRQ ELEREEEALA SKRFRAERQD
61NKENWLHSQQ REAEQRAALA RLAGQLESMN DVEELTALLR SAGEYEERKL IRAAIRRVRA
121QEIEAATLAG RLYSGRPNSG SREDSKGLAA HRLEQCEVPE REEQEQQAEV SKPTPTPEGT
181SQDVTTVTLL LRAPPGSTSS SPASPSSSPT PASPEPPLEP AEAQCLTAEV PGSPEPPPSP
241PKTTSPEPQE SPTLPSTEGQ VVNKLLSGPK ETPAAQSPHR GPSDTKRADV AGPRPCQRSL
301SVLSPRQPAQ NRESTPLASG PSSFQRAGSV RDRVHKFTSD SPMAARLQDG TPQAALSPLT
361PARLLGPSLT STTPASSSSG SSSRGPSDTS SRFSKEQRGV AQPLAQLRSC PQEEGPRGRG
421LAARPLENRA GGPVARSEEP GAPLPVAVGT AEPGGSMKTT FTIEIKDGRG QASTGRVLLP
481TGNQRAELTL GLRAPPTLLS TSSGGKSTIT RVNSPGTLAR LGSVTHVTSF SHAPPSSRGG
541CSIKMEAEPA EPLAAAVEAA NGAEQTRVNK APEGRSPLSA EELMTIEDEG VLDKMLDQST
601DFEERKLIRA ALRELRQRKR DQRDKERERR LQEARGRPGE GRGNTATETT TRHSQRAADG
661SAVSTVTKTE RLVHSNDGTR TARTTTVESS FVRRSENGSG STMMQTKTFS SSSSSKKMGS
721IFDREDQASP RAGSLAALEK RQAEKKKELM KAQSLPKTSA SQARKAMIEK LEKEGAAGSP
781GGPRAAVQRS TSFGVPNANS IKQMLLDWCR AKTRGYEHVD IQNFSSSWSD GMAFCALVHN
841FFPEAFDYGQ LSPQNRRQNF EVAFSSAETH ADCPQLLDTE DMVRLREPDW KMLVDCVPLV
901EVDDMMIMGK KPDPKCVFTY VQSLYNHLRR HELRLRGKNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

940 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.