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Human SMG6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SMG6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SMG6 antibodies →
$220.00each
Catalog Number: LS-G82823-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EST1A(SMG6) (NM_017575) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf31 | EST1-like protein A | Ever shorter telomeres 1A | KIAA0732 | Smg-6 homolog | SMG6 | SMG-6 | Telomerase subunit EST1A | EST1A | HSMG5/7a
Origin Species
Human
NCBI GenBank
NM_017575
SwissProt
Q86US8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
160.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEGLERVRI SASELRGILA TLAPQAGSRE NMKELKEARP RKDNRRPDLE IYKPGLSRLR
61NKPKIKEPPG SEEFKDEIVN DRDCSAVENG TQPVKDVCKE LNNQEQNGPI DPENNRGQES
121FPRTAGQEDR SLKIIKRTKK PDLQIYQPGR RLQTVSKESA SRVEEEEVLN QVEQLRVEED
181ECRGNVAKEE VANKPDRAEI EKSPGGGRVG AAKGEKGKRM GKGEGVRETH DDPARGRPGS
241AKRYSRSDKR RNRYRTRSTS SAGSNNSAEG AGLTDNGCRR RRQDRTKERP RLKKQVSVSS
301TDSLDEDRID EPDGLGPRRS SERKRHLERN WSGRGEGEQK NSAKEYRGTL RVTFDAEAMN
361KESPMVRSAR DDMDRGKPDK GLSSGGKGSE KQESKNPKQE LRGRGRGILI LPAHTTLSVN
421SAGSPESAPL GPRLLFGSGS KGSRSWGRGG TTRRLWDPNN PDQKPALKTQ TPQLHFLDTD
481DEVSPTSWGD SRQAQASYYK FQNSDNPYYY PRTPGPASQY PYTGYNPLQY PVGPTNGVYP
541GPYYPGYPTP SGQYVCSPLP TSTMSPEEVE QHMRNLQQQE LHRLLRVADN QELQLSNLLS
601RDRISPEGLE KMAQLRAELL QLYERCILLD IEFSDNQNVD QILWKNAFYQ VIEKFRQLVK
661DPNVENPEQI RNRLLELLDE GSDFFDSLLQ KLQVTYKFKL EDYMDGLAIR SKPLRKTVKY
721ALISAQRCMI CQGDIARYRE QASDTANYGK ARSWYLKAQH IAPKNGRPYN QLALLAVYTR
781RKLDAVYYYM RSLAASNPIL TAKESLMSLF EETKRKAEQM EKKQHEEFDL SPDQWRKGKK
841STFRHVGDDT TRLEIWIHPS HPRSSQGTES GKDSEQENGL GSLSPSDLNK RFILSFLHAH
901GKLFTRIGME TFPAVAEKVL KEFQVLLQHS PSPIGSTRML QLMTINMFAV HNSQLKDCFS
961EECRSVIQEQ AAALGLAMFS LLVRRCTCLL KESAKAQLSS PEDQDDQDDI KVSSFVPDLK
1021ELLPSVKVWS DWMLGYPDTW NPPPTSLDLP SHVAVDVWST LADFCNILTA VNQSEVPLYK
1081DPDDDLTLLI LEEDRLLSGF VPLLAAPQDP CYVEKTSDKV IAADCKRVTV LKYFLEALCG
1141QEEPLLAFKG GKYVSVAPVP DTMGKEMGSQ EGTRLEDEEE DVVIEDFEED SEAEGSGGED
1201DIRELRAKKL ALARKIAEQQ RRQEKIQAVL EDHSQMRQME LEIRPLFLVP DTNGFIDHLA
1261SLARLLESRK YILVVPLIVI NELDGLAKGQ ETDHRAGGYA RVVQEKARKS IEFLEQRFES
1321RDSCLRALTS RGNELESIAF RSEDITGQLG NNDDLILSCC LHYCKDKAKD FMPASKEEPI
1381RLLREVVLLT DDRNLRVKAL TRNVPVRDIP AFLTWAQVG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1419 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.