SMAP1 Antibody
Catalog Number: LS-C378056
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SMAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G84112-20
Catalog Number: LS-G84112
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SMAP1 (NM_001044305) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SMAP1 | Small ArfGAP 1 | SMAP-1
- Origin Species
- Human
- NCBI GenBank
- NM_021940
- SwissProt
- Q8IYB5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH61RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR121DKYEKKKYYD KNAIAITNIS SSDAPLQPLV SSPSLQAAVD KNKLEKEKEK KKEEKKREKE181PEKPAKPLTA EKLQKKDQQL EPKKSTSPKK AAEPTVDLLG LDGPAVAPVT NGNTTVPPLN241DDLDIFGPMI SNPLPATVMP PAQGTPSAPA AATLSTVTSG DLDLFTEQTT KSEEVAKKQL301SKDSILSLYG TGTIQQQSTP GVFMGPTNIP FTSQAPAAFQ GFPSMGVPVP AAPGLIGNVM361GQSPSMMVGM PMPNGFMGNA QTGVMPLPQN VVGHQGGMVG QMGAPQSKFG LPQAQQPQWS421LSQMNQQMAG MSISSATPTA GFGQPSSTTA GWSGSSSGQT LSTQLWKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
467 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
