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Human SLC9A8 / NHE8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SLC9A8 / NHE8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G86573-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SLC9A8 (NM_015266) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0939 | Na(+)/H(+) exchanger 8 | NHE8 | SLC9A8 | NHE-8 | Sodium/hydrogen exchanger 8
Origin Species
Human
NCBI GenBank
AB023156
SwissProt
Q9Y2E8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGEKMAEEER FPNTTHEGFN VTLHTTLVVT TKLVLPTPGK PILPVQTGEQ AQQEEQSSGM
61TIFFSLLVLA ICIILVHLLI RYRLHFLPES VAVVSLGILM GAVIKIIEFK KLANWKEEEM
121FRPNMFFLLL LPPIIFESGY SLHKGNFFQN IGSITLFAVF GTAISAFVVG GGIYFLGQAD
181VISKLNMTDS FAFGSLISAV DPVATIAIFN ALHVDPVLNM LVFGESILND AVSIVLTNTA
241EGLTRKNMSD VSGWQTFLQA LDYFLKMFFG SAALGTLTGL ISALVLKHID LRKTPSLEFG
301MMIIFAYLPY GLAEGISLSG IMAILFSGIV MSHYTHHNLS PVTQILMQQT LRTVAFLCET
361CVFAFLGLSI FSFPHKFEIS FVIWCIVLVL FGRAVNIFPL SYLLNFFRDH KITPKMMFIM
421WFSGLRGAIP YALSLHLDLE PMEKRQLIGT TTIVIVLFTI LLLGGSTMPL IRLMDIEDAK
481AHRRNKKDVN LSKTEKMGNT VESEHLSELT EEEYEAHYIR RQDLKGFVWL DAKYLNPFFT
541RRLTQEDLHH GRIQMKTLTN KWYEEVRQGP SGSEDDEQEL L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.