Human SLC45A1 ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F12551-1
1 plate / $683.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SLC45A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G94397-20
Catalog Number: LS-G94397
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SLC45A1 (NM_001080397) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNB5 | DNb-5 | Deleted in neuroblastoma 5 | PAST-A | SLC45A1
- Origin Species
- Human
- SwissProt
- Q9Y2W3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIPAASSTPP GDALFPSVAP QDFWRSQVTG YSGSVTRHLS HRANNFKRHP KRRKCIRPSP61PPPPNTPCPL ELVDFGDLHP QRSFRELLFN GCILFGIEFS YAMETAYVTP VLLQMGLPDQ121LYSLVWFISP ILGFLLQPLL GAWSDRCTSR FGRRRPFILV LAIGALLGLS LLLNGRDIGI181ALADVTGNHK WGLLLTVCGV VLMDFSADSA DNPSHAYMMD VCSPADQDRG LNIHALLAGL241GGGFGYVVGG IHWDKTGFGR ALGGQLRVIY LFTAVTLSVT TVLTLVSIPE RPLRPPSEKR301AAMKSPSLPL PPSPPVLPEE GPGDSLPSHT ATNFSSPISP PSPLTPKYGS FISRDSSLTG361ISEFASSFGT ANIDSVLIDC FTGGHDSYLA IPGSVPRPPI SVSFPRAPDG FYRQDRGLLE421GREGALTSGC DGDILRVGSL DTSKPRSSGI LKRPQTLAIP DAAGGGGPET SRRRNVTFSQ481QVANILLNGV KYESELTGSS ERAEQPLSVG RLCSTICNMP KALRTLCVNH FLGWLSFEGM541LLFYTDFMGE VVFQGDPKAP HTSEAYQKYN SGVTMGCWGM CIYAFSAAFY SAILEKLEEF601LSVRTLYFIA YLAFGLGTGL ATLSRNLYVV LSLCITYGIL FSTLCTLPYS LLCDYYQSKK661FAGSSADGTR RGMGVDISLL SCQYFLAQIL VSLVLGPLTS AVGSANGVMY FSSLVSFLGC721LYSSLFVIYE IPPSDAADEE HRPLLLNVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
748 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
