SKIV2L / SKI2 Antibody
Catalog Number: LS-C676492
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SKIV2L / SKI2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76393-20
Catalog Number: LS-G76393
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Helicase SKI2W(SKIV2L) (NM_006929) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 170A | DDX13 | Helicase SKI2W | HLP | SKI2 | SKI2W | SKIV2L | SKIV2 | Helicase-like protein | THES2
- Origin Species
- Human
- NCBI GenBank
- NM_006929
- SwissProt
- Q15477
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 137.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMETERLVLP PPDPLDLPLR AVELGCTGHW ELLNLPGAPE SSLPHGLPPC APDLQQEAEQ61LFLSSPAWLP LHGVEHSARK WQRKTDPWSL LAVLGAPVPS DLQAQRHPTT GQILGYKEVL121LENTNLSATT SLSLRRPPGP ASQSLWGNPT RYPFWPGGMD EPTITDLNTR EEAEEEIDFE181KDLLTIPPGF KKGMDFAPKD CPTPAPGLLS LSCLLEPLDL GGGDEDENEA VGQPGGPRGD241TVSASPCSAP LARASSLEDL VLKEASTAVS TPEAPEPPSQ EQWAIPVDAT SPVGDFYRLI301PQPAFQWAFE PDVFQKQAIL HLERHDSVFV AAHTSAGKTV VAEYAIALAQ KHMTRTIYTS361PIKALSNQKF RDFRNTFGDV GLLTGDVQLH PEASCLIMTT EILRSMLYSG SDVIRDLEWV421IFDEVHYIND VERGVVWEEV LIMLPDHVSI ILLSATVPNA LEFADWIGRL KRRQIYVIST481VTRPVPLEHY LFTGNSSKTQ GELFLLLDSR GAFHTKGYYA AVEAKKERMS KHAQTFGAKQ541PTHQGGPAQD RGVYLSLLAS LRTRAQLPVV VFTFSRGRCD EQASGLTSLD LTTSSEKSEI601HLFLQRCLAR LRGSDRQLPQ VLHMSELLNR GLGVHHSGIL PILKEIVEML FSRGLVKVLF661ATETFAMGVN MPARTVVFDS MRKHDGSTFR DLLPGEYVQM AGRAGRRGLD PTGTVILLCK721GRVPEMADLH RMMMGKPSQL QSQFRLTYTM ILNLLRVDAL RVEDMMKRSF SEFPSRKDSK781AHEQALAELT KRLGALEEPD MTGQLVDLPE YYSWGEELTE TQHMIQRRIM ESVNGLKSLS841AGRVVVVKNQ EHHNALGVIL QVSSNSTSRV FTTLVLCDKP LSQDPQDRGP ATAEVPYPDD901LVGFKLFLPE GPCDHTVVKL QPGDMAAITT KVLRVNGEKI LEDFSKRQQP KFKKDPPLAA961VTTAVQELLR LAQAHPAGPP TLDPVNDLQL KDMSVVEGGL RARKLEELIQ GAQCVHSPRF1021PAQYLKLRER MQIQKEMERL RFLLSDQSLL LLPEYHQRVE VLRTLGYVDE VGTVKLAGRV1081ACAMSSHELL LTELMFDNAL STLRPEEIAA LLSGLVCQSP GDAGDQLPNT LKQGIERVRA1141VAKRIGEVQV ACGLNQTVEE FVGELNFGLV EVVYEWARGM PFSELAGLSG TPEGLVVRCI1201QRLAEMCRSL RGAARLVGEP VLGAKMETAA TLLRRDIVFA ASLYTQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1246 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
