SHH / Sonic Hedgehog Antibody
Catalog Number: LS-C405795
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SHH / Sonic Hedgehog Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81212-20
Catalog Number: LS-G81212
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Sonic Hedgehog(SHH) (NM_000193) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | SHH | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
- Origin Species
- Human
- NCBI GenBank
- NM_000193
- SwissProt
- Q15465
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEMLLLARC LLLVLVSSLL VCSGLACGPG RGFGKRRHPK KLTPLAYKQF IPNVAEKTLG61ASGRYEGKIS RNSERFKELT PNYNPDIIFK DEENTGADRL MTQRCKDKLN ALAISVMNQW121PGVKLRVTEG WDEDGHHSEE SLHYEGRAVD ITTSDRDRSK YGMLARLAVE AGFDWVYYES181KAHIHCSVKA ENSVAAKSGG CFPGSATVHL EQGGTKLVKD LSPGDRVLAA DDQGRLLYSD241FLTFLDRDDG AKKVFYVIET REPRERLLLT AAHLLFVAPH NDSATGEPEA SSGSGPPSGG301ALGPRALFAS RVRPGQRVYV VAERDGDRRL LPAAVHSVTL SEEAAGAYAP LTAQGTILIN361RVLASCYAVI EEHSWAHRAF APFRLAHALL AALAPARTDR GGDSGGGDRG GGGGRVALTA421PGAADAPGAG ATAGIHWYSQ LLYQIGTWLL DSEALHPLGM AVKSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
