SGCE Antibody
Catalog Number: LS-C333988
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SGCE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G80429-20
Catalog Number: LS-G80429
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SGCE (NM_001099400) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DYT11 | Dystonia 11 | myoclonic | Epsilon-SG | ESG | Sarcoglycan | epsilon | Epsilon-sarcoglycan | SGCE
- Origin Species
- Human
- NCBI GenBank
- NM_003919
- SwissProt
- O43556
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQLPRWWELG DPCAWTGQGR GTRRMSPATT GTFLLTVYSI FSKVHSDRSV YPSAGVLFVH61VLEREYFKGE FPPYPKPGEI SNDPITFNTN LMGYPDRPGW LRYIQRTPYS DGVLYGSPTA121ENVGKPTIIE ITAYNRRTFE TARHNLIINI MSAEDFPLPY QAEFFIKNMN VEEMLASEVL181GDFLGAVKNV WQPERLNAIN ITSALDRGGR VPLPINDLKE GVYVMVGADV PFSSCLREVE241NPQNQLRCSQ EMEPVITCDK KFRTQFYIDW CKISLVDKTK QVSTYQEVIR GEGILPDGGE301YKPPSDSLKS RDYYTDFLIT LAVPSAVALV LFLILAYIMC CRREGVIQLV HHSAIQKSTK361ELRDMSKNRE IAWPLSTLPV FHPVTGEIIH PLHTDNYDST NMPLMQTQQN LPHQTQIPQQ421QTTGDFRLTT FQRFEVNGIP EERKLTEAMN LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
451 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
