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Human SEMA5B / Semaphorin 5B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SEMA5B / Semaphorin 5B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SEMA5B antibodies →
$153.00each
Catalog Number: LS-G88555-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEMA5B (NM_001031702) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1445 | SEMA5B | SEMG | SEMAG | Semaphorin-5B
Origin Species
Human
NCBI GenBank
AB040878
SwissProt
Q9P283

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
125.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPCGFSPSPV AHHLVPGPPD TPAQQLRCGW TVGGWLLSLV RGLLPCLPPG ARTAEGPIMV
61LAGPLAVSLL LPSLTLLVSH LSSSQDVSSE PSSEQQLCAL SKHPTVAFED LQPWVSNFTY
121PGARDFSQLA LDPSGNQLIV GARNYLFRLS LANVSLLQAT EWASSEDTRR SCQSKGKTEE
181ECQNYVRVLI VAGRKVFMCG TNAFSPMCTS RQVGNLSRTI EKINGVARCP YDPRHNSTAV
241ISSQGELYAA TVIDFSGRDP AIYRSLGSGP PLRTAQYNSK WLNEPNFVAA YDIGLFAYFF
301LRENAVEHDC GRTVYSRVAR VCKNDVGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ
361SAFHLPEQDL IYGVFTTNVN SIAASAVCAF NLSAISQAFN GPFRYQENPR AAWLPIANPI
421PNFQCGTLPE TGPNENLTER SLQDAQRLFL MSEAVQPVTP EPCVTQDSVR FSHLVVDLVQ
481AKDTLYHVLY IGTESGTILK ALSTASRSLH GCYLEELHVL PPGRREPLRS LRILHSARAL
541FVGLRDGVLR VPLERCAAYR SQGACLGARD PYCGWDGKQQ RCSTLEDSSN MSLWTQNITA
601CPVRNVTRDG GFGPWSPWQP CEHLDGDNSG SCLCRARSCD SPRPRCGGLD CLGPAIHIAN
661CSRNGAWTPW SSWALCSTSC GIGFQVRQRS CSNPAPRHGG RICVGKSREE RFCNENTPCP
721VPIFWASWGS WSKCSSNCGG GMQSRRRACE NGNSCLGCGV EFKTCNPEGC PEVRRNTPWT
781PWLPVNVTQG GARQEQRFRF TCRAPLADPH GLQFGRRRTE TRTCPADGSG SCDTDALVED
841LLRSGSTSPH TVSGGWAAWG PWSSCSRDCE LGFRVRKRTC TNPEPRNGGL PCVGDAAEYQ
901DCNPQACPVR GAWSCWTSWS PCSASCGGGH YQRTRSCTSP APSPGEDICL GLHTEEALCA
961TQACPEGWSP WSEWSKCTDD GAQSRSRHCE ELLPGSSACA GNSSQSRPCP YSEIPVILPA
1021SSMEEATGCA GFNLIHLVAT GISCFLGSGL LTLAVYLSCQ HCQRQSQEST LVHPATPNHL
1081HYKGGGTPKN EKYTPMEFKT LNKNNLIPDD RANFYPLQQT NVYTTTYYPS PLNKHSFRPE
1141ASPGQRCFPN S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1151 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.