Human SEC61A2 Protein (Recombinant GST)
Catalog Number: LS-G34217
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SEC61A2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G90832-20
Catalog Number: LS-G90832
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEC61A2 (NM_001142627) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Sec61 alpha form 2 | SEC61A2 | Sec61 alpha-2
- Origin Species
- Human
- NCBI GenBank
- NM_018144
- SwissProt
- Q9H9S3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD61PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG121MIITIGQAIV YVMTGMYGDP AEMGAGICLL IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS181LFIATNICET IVWKAFSPTT INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP241NLMNLIATVF VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN301LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES MGAIFEDPVH361VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRDTSMV HELNRPKVKL421RRWKGEGRHF TKRILFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
437 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
