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Human SBNO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SBNO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SBNO1 antibodies →
$153.00each
Catalog Number: LS-G90855-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SBNO1 (NM_018183) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Monocyte protein 3 | MOP-3 | SBNO1 | Sno | MOP3
Origin Species
Human
NCBI GenBank
NM_018183

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
154 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVEPGQDLLL AALSESGISP NDLFDIDGGD AGLATPMPTP SVQQSVPLSA LELGLETEAA
61VPVKQEPETV PTPALLNVRQ QPPSTTTFVL NQINHLPPLG STIVMTKTPP VTTNRQTITL
121TKFIQTTAST RPSVSAPTVR NAMTSAPSKD QVQLKDLLKN NSLNELMKLK PPANIAQPVA
181TAATDVSNGT VKKESSNKEG ARMWINDMKM RSFSPTMKVP VVKEDDEPEE EDEEEMGHAE
241TYAEYMPIKL KIGLRHPDAV VETSSLSSVT PPDVWYKTSI SEETIDNGWL SALQLEAITY
301AAQQHETFLP NGDRAGFLIG DGAGVGKGRT IAGIIYENYL LSRKRALWFS VSNDLKYDAE
361RDLRDIGAKN ILVHSLNKFK YGKISSKHNG SVKKGVIFAT YSSLIGESQS GGKYKTRLKQ
421LLHWCGDDFD GVIVFDECHK AKNLCPVGSS KPTKTGLAVL ELQNKLPKAR VVYASATGAS
481EPRNMAYMNR LGIWGEGTPF REFSDFIQAV ERRGVGAMEI VAMDMKLRGM YIARQLSFTG
541VTFKIEEVLL SQSYVKMYNK AVKLWVIARE RFQQAADLID AEQRMKKSMW GQFWSAHQRF
601FKYLCIASKV KRVVQLAREE IKNGKCVVIG LQSTGEARTL EALEEGGGEL NDFVSTAKGV
661LQSLIEKHFP APDRKKLYSL LGIDLTAPSN NSSPRDSPCK ENKIKKRKGE EITREAKKAR
721KVGGLTGSSS DDSGSESDAS DNEESDYESS KNMSSGDDDD FNPFLDESNE DDENDPWLIR
781KDHKKNKEKK KKKSIDPDSI QSALLASGLG SKRPSFSSTP VISPAPNSTP ANSNTNSNSS
841LITSQDAVER AQQMKKDLLD KLEKLAEDLP PNTLDELIDE LGGPENVAEM TGRKGRVVSN
901DDGSISYESR SELDVPVEIL NITEKQRFMD GDKNIAIISE AASSGISLQA DRRAKNQRRR
961VHMTLELPWS ADRAIQQFGR THRSNQVTAP EYVFLISELA GEQRFASIVA KRLESLGALT
1021HGDRRATESR DLSRFNFDNK YGRNALEIVM KSIVNLDSPM VSPPPDYPGE FFKDVRQGLI
1081GVGLINVEDR SGILTLDKDY NNIGKFLNRI LGMEVHQQNA LFQYFADTLT AVVQNAKKNG
1141RYDMGILDLG SGDEKVRKSD VKKFLTPGYS TSGHVELYTI SVERGMSWEE ATKIWAELTG
1201PDDGFYLSLQ IRNNKKTAIL VKEVNPKKKL FLVYRPNTGK QLKLEIYADL KKKYKKVVSD
1261DALMHWLDQY NSSADTCTHA YWRGNCKKAS LGLVCEIGLR CRTYYVLCGS VLSVWTKVEG
1321VLASVSGTNV KMQIVRLRTE DGQRIVGLII PANCVSPLVN LLSTSDQSQQ LAVQQKQLWQ
1381QHHPQSITNL SNA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1393 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.