SASH1 Antibody
Catalog Number: LS-C832636
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SASH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95285-20
Catalog Number: LS-G95285
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SASH1 (NM_015278) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SH3D6A | PEPE1 | RP3-323M4.1 | DJ323M4 | DJ323M4.1 | KIAA0790 | SASH1
- Origin Species
- Human
- NCBI GenBank
- NM_015278
- SwissProt
- O94885
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 136.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDAGAAGPG PEPEPEPEPE PEPAPEPEPE PKPGAGTSEA FSRLWTDVMG ILDGSLGNID61DLAQQYADYY NTCFSDVCER MEELRKRRVS QDLEVEKPDA SPTSLQLRSQ IEESLGFCSA121VSTPEVERKN PLHKSNSEDS SVGKGDWKKK NKYFWQNFRK NQKGIMRQTS KGEDVGYVAS181EITMSDEERI QLMMMVKEKM ITIEEALARL KEYEAQHRQS AALDPADWPD GSYPTFDGSS241NCNSREQSDD ETEESVKFKR LHKLVNSTRR VRKKLIRVEE MKKPSTEGGE EHVFENSPVL301DERSALYSGV HKKPLFFDGS PEKPPEDDSD SLTTSPSSSS LDTWGAGRKL VKTFSKGESR361GLIKPPKKMG TFFSYPEEEK AQKVSRSLTE GEMKKGLGSL SHGRTCSFGG FDLTNRSLHV421GSNNSDPMGK EGDFVYKEVI KSPTASRISL GKKVKSVKET MRKRMSKKYS SSVSEQDSGL481DGMPGSPPPS QPDPEHLDKP KLKAGGSVES LRSSLSGQSS MSGQTVSTTD SSTSNRESVK541SEDGDDEEPP YRGPFCGRAR VHTDFTPSPY DTDSLKLKKG DIIDIISKPP MGTWMGLLNN601KVGTFKFIYV DVLSEDEEKP KRPTRRRRKG RPPQPKSVED LLDRINLKEH MPTFLFNGYE661DLDTFKLLEE EDLDELNIRD PEHRAVLLTA VELLQEYDSN SDQSGSQEKL LVDSQALSGC721SPRDSGCYES SENLENGKTR KASLLSAKSS TEPSLKSFSR NQLGNYPTLP LMKSGDALKQ781GQEEGRLGGG LAPDTSKSCD PPGVTGLNKN RRSLPVSICR SCETLEGPQT VDTWPRSHSL841DDLQVEPGAE QDVPTEVTEP PPQIVPEVPQ KTTASSTKAQ PLEQDSAVDN ALLLTQSKRF901SEPQKLTTKK LEGSIAASGR GLSPPQCLPR NYDAQPPGAK HGLARTPLEG HRKGHEFEGT961HHPLGTKEGV DAEQRMQPKI PSQPPPVPAK KSRERLANGL HPVPMGPSGA LPSPDAPCLP1021VKRGSPASPT SPSDCPPALA PRPLSGQAPG SPPSTRPPPW LSELPENTSL QEHGVKLGPA1081LTRKVSCARG VDLETLTENK LHAEGIDLTE EPYSDKHGRC GIPEALVQRY AEDLDQPERD1141VAANMDQIRV KQLRKQHRMA IPSGGLTEIC RKPVSPGCIS SVSDWLISIG LPMYAGTLST1201AGFSTLSQVP SLSHTCLQEA GITEERHIRK LLSAARLFKL PPGPEAMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1247 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
