LSBio
Human RUSC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RUSC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RUSC2 antibodies →
$220.00each
Catalog Number: LS-G79773-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RUSC2 (NM_014806) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Iporin | Interacting protein of Rab1 | RUSC2 | KIAA0375
Origin Species
Human
NCBI GenBank
AB002373
SwissProt
Q8N2Y8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
161 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSPPKLTGE TLIVHHIPLV HCQVPDRQCC GGAGGGGGST RPNPFCPPEL GITQPDQDLG
61QADSLLFSSL HSAPGGTARS IDSTKSRSRD GRGPGAPKRH NPFLLQEGVG EPGLGDLYDD
121SIGDSATQQS FHLHGTGQPN FHLSSFQLPP SGPRVGRPWG TTRSRAGVVE GQEQEPVMTL
181DTQQCGTSHC CRPELEAETM ELDECGGPGG SGSGGGASDT SGFSFDQEWK LSSDESPRNP
241GCSGSGDQHC RCSSTSSQSE AADQSMGYVS DSSCNSSDGV LVTFSTLYNK MHGTPRANLN
301SAPQSCSDSS FCSHSDPGAF YLDLQPSPFE SKMSYESHHP ESGGREGGYG CPHASSPELD
361ANCNSYRPHC EPCPAVADLT ACFQSQARLV VATQNYYKLV TCDLSSQSSP SPAGSSITSC
421SEEHTKISPP PGPGPDPGPS QPSEYYLFQK PEVQPEEQEA VSSSTQAAAA VGPTVLEGQV
481YTNTSPPNLS TGRQRSRSYD RSLQRSPPVR LGSLERMLSC PVRLSEGPAA MAGPGSPPRR
541VTSFAELAKG RKKTGGSGSP PLRVSVGDSS QEFSPIQEAQ QDRGAPLDEG TCCSHSLPPM
601PLGPGMDLLG PDPSPPWSTQ VCQGPHSSEM PPAGLRATGQ GPLAQLMDPG PALPGSPANS
661HTQRDARARA DGGGTESRPV LRYSKEQRPT TLPIQPFVFQ HHFPKQLAKA RALHSLSQLY
721SLSGCSRTQQ PAPLAAPAAQ VSVPAPSGEP QASTPRATGR GARKAGSEPE TSRPSPLGSY
781SPIRSVGPFG PSTDSSASTS CSPPPEQPTA TESLPPWSHS CPSAVRPATS QQPQKEDQKI
841LTLTEYRLHG TGSLPPLGSW RSGLSRAESL ARGGGEGSMA TRPSNANHLS PQALKWREYR
901RKNPLGPPGL SGSLDRRSQE ARLARRNPIF EFPGSLSAAS HLNCRLNGQA VKPLPLTCPD
961FQDPFSLTEK PPAEFCLSPD GSSEAISIDL LQKKGLVKAV NIAVDLIVAH FGTSRDPGVK
1021AKLGNSSVSP NVGHLVLKYL CPAVRAVLED GLKAFVLDVI IGQRKNMPWS VVEASTQLGP
1081STKVLHGLYN KVSQFPELTS HTMRFNAFIL GLLNIRSLEF WFNHLYNHED IIQTHYQPWG
1141FLSAAHTVCP GLFEELLLLL QPLALLPFSL DLLFQHRLLQ SGQQQRQHKE LLRVSQDLLL
1201SAHSTLQLAR ARGQEGPGDV DRAAQGERVK GVGASEGGEE EEEEEETEEV AEAAGGSGRA
1261RWARGGQAGW WYQLMQSSQV YIDGSIEGSR FPRGSSNSSS EKKKGAGGGG PPQAPPPREG
1321VVEGAEACPA SEEALGRERG WPFWMGSPPD SVLAELRRSR EREGPAASPA ENEEGASEPS
1381PGGIKWGHLF GSRKAQREAR PTNRLPSDWL SLDKSMFQLV AQTVGSRREP EPKESLQEPH
1441SPALPSSPPC EVQALCHHLA TGPGQLSFHK GDILRVLGRA GGDWLRCSRG PDSGLVPLAY
1501VTLTPTPSPT PGSSQN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.