RNF6 Antibody
Catalog Number: LS-C749575
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RNF6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G89449-20
Catalog Number: LS-G89449
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RNF6 (NM_183044) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SPG2 | RING-H2 protein RNF-6 | RNF6
- Origin Species
- Human
- NCBI GenBank
- NM_005977
- SwissProt
- Q9Y252
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNQSRSRSDG GSEETLPQDH NHHENERRWQ QERLHREEAY YQFINELNDE DYRLMRDHNL61LGTPGEITSE ELQQRLDGVK EQLASQPDLR DGTNYRDSEV PRESSHEDSL LEWLNTFRRT121GNATRSGQNG NQTWRAVSRT NPNNGEFRFS LEIHVNHENR GFEIHGEDYT DIPLSDSNRD181HTANRQQRST SPVARRTRSQ TSVNFNGSSS NIPRTRLASR GQNPAEGSFS TLGRLRNGIG241GAAGIPRANA SRTNFSSHTN QSGGSELRQR EGQRFGAAHV WENGARSNVT VRNTNQRLEP301IRLRSTSNSR SRSPIQRQSG TVYHNSQRES RPVQQTTRRS VRRRGRTRVF LEQDRERERR361GTAYTPFSNS RLVSRITVEE GEESSRSSTA VRRHPTITLD LQVRRIRPGE NRDRDSIANR421TRSRVGLAEN TVTIESNSGG FRRTISRLER SGIRTYVSTI TVPLRRISEN ELVEPSSVAL481RSILRQIMTG FGELSSLMEA DSESELQRNG QHLPDMHSEL SNLGTDNNRS QHREGSSQDR541QAQGDSTEMH GENETTQPHT RNSDSRGGRQ LRNPNNLVET GTLPILRLAH FFLLNESDDD601DRIRGLTKEQ IDNLSTRHYE HNSIDSELGK ICSVCISDYV TGNKLRQLPC MHEFHIHCID661RWLSENCTCP ICRQPVLGSN IANNGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
