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Human RNF214 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RNF214 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RNF214 antibodies →
$220.00each
Catalog Number: LS-G95272-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RNF214 (NM_001077239) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RNF214 | Ring finger protein 214
Origin Species
Human
NCBI GenBank
NM_001077239
SwissProt
Q8ND24

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASEVAGVV ANAPSPPESS SLCASKSDEG LPDGLSTKDS AQKQKNSPLL SVSSQTITKE
61NNRNVHLEHS EQNPGSSAGD TSAAHQVVLG ENLIATALCL SGSGSQSDLK DVASTAGEEG
121DTSLRESLHP VTRSLKAGCH TKQLASRNCS EEKSPQTSIL KEGNRDTSLD FRPVVSPANG
181VEGVRVDQDD DQDSSSLKLS QNIAVQTDFK TADSEVNTDQ DIEKNLDKMM TERTLLKERY
241QEVLDKQRQV ENQLQVQLKQ LQQRREEEMK NHQEILKAIQ DVTIKREETK KKIEKEKKEF
301LQKEQDLKAE IEKLCEKGRR EVWEMELDRL KNQDGEINRN IMEETERAWK AEILSLESRK
361ELLVLKLEEA EKEAELHLTY LKSTPPTLET VRSKQEWETR LNGVRIMKKN VRDQFNSHIQ
421LVRNGAKLSS LPQIPTPTLP PPPSETDFML QVFQPSPSLA PRMPFSIGQV TMPMVMPSAD
481PRSLSFPILN PALSQPSQPS SPLPGSHGRN SPGLGSLVSP HGPHMPPAAS IPPPPGLGGV
541KASAETPRPQ PVDKLEKILE KLLTRFPQCN KAQMTNILQQ IKTARTTMAG LTMEELIQLV
601AARLAEHERV AASTQPLGRI RALFPAPLAQ ISTPMFLPSA QVSYPGRSSH APATCKLCLM
661CQKLVQPSEL HPMACTHVLH KECIKFWAQT NTNDTCPFCP TLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

703 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.