RNF174 / MARCH4 Antibody
Catalog Number: LS-C824060-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RNF174 / MARCH4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93196-20
Catalog Number: LS-G93196
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MARCH4 (NM_020814) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MARCH4 | RING finger protein 174 | KIAA1399 | MARCH-IV | RNF174
- Origin Species
- Human
- SwissProt
- Q9P2E8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLMPLCGLLW WWWCCCSGWY CYGLCAPAPQ MLRHQGLLKC RCRMLFNDLK VFLLRRPPQA61PLPMHGDPQP PGLAANNTLP ALGAGGWAGW RGPREVVGRE PPPVPPPPPL PPSSVEDDWG121GPATEPPASL LSSASSDDFC KEKTEDRYSL GSSLDSGMRT PLCRICFQGP EQGELLSPCR181CDGSVKCTHQ PCLIKWISER GCWSCELCYY KYHVIAISTK NPLQWQAISL TVIEKVQVAA241AILGSLFLIA SISWLIWSTF SPSARWQRQD LLFQICYGMY GFMDVVCIGL IIHEGPSVYR301IFKRWQAVNQ QWKVLNYDKT KDLEDQKAGG RTNPRTSSST QANIPSSEEE TAGTPAPEQG361PAQAAGHPSG PLSHHHCAYT ILHILSHLRP HEQRSPPGSS RELVMRVTTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
410 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
