RNF149 Antibody
Catalog Number: LS-C833513
3 sizes / from $338.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RNF149 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G82737-20
Catalog Number: LS-G82737
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RNF149 (NM_173647) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RNF149 | Ring finger protein 149 | DNAPTP2
- Origin Species
- Human
- NCBI GenBank
- AA203416
- SwissProt
- Q8NC42
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWRRREASV GARGVLALAL LALALCVPGA RGRALEWFSA VVNIEYVDPQ TNLTVWSVSE61SGRFGDSSPK EGAHGLVGVP WAPGGDLEGC APDTRFFVPE PGGRGAAPWV ALVARGGCTF121KDKVLVAARR NASAVVLYNE ERYGNITLPM SHAGTGNIVV IMISYPKGRE ILELVQKGIP181VTMTIGVGTR HVQEFISGQS VVFVAIAFIT MMIISLAWLI FYYIQRFLYT GSQIGSQSHR241KETKKVIGQL LLHTVKHGEK GIDVDAENCA VCIENFKVKD IIRILPCKHI FHRICIDPWL301LDHRTCPMCK LDVIKALGYW GEPGDVQEMP APESPPGRDP AANLSLALPD DDGSDESSPP361SASPAESEPQ CDPSFKGDAG ENTALLEAGR SDSRHGGPISTRTRPLEQKLISEEDLAANDILDYKDDDDKV
400 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
