RHOT1 Antibody
Catalog Number: LS-C668793
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RHOT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G90920-20
Catalog Number: LS-G90920
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MIRO1(RHOT1) (NM_001033568) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHT1 | HMiro-1 | MIRO-1 | Mitochondrial Rho GTPase 1 | MIRO1 | Mitochondrial Rho 1 | Ras homolog family member T1 | RHOT1
- Origin Species
- Human
- NCBI GenBank
- NM_018307
- SwissProt
- Q8IXI2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE61AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS121DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE181MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG241VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE301LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ361GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR421CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE481FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI541SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP EDHYRDRLSR DMGHTDRIEN601LRKIWVFLKT AFHARLRCMC TCNRCTFCIC QNFLNSDLLQ SVKNKIFTAV LNRHVTQADL661KSSTFWLRAS FGATVFAVLG FAMYKALLKQ RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
