Human RDH16 ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67046-1
1 plate / $683.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RDH16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92491-20
Catalog Number: LS-G92491
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Retinol dehydrogenase 16(RDH16) (NM_003708) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RDH16 | Retinol dehydrogenase 16 | RODH-4 | RODH4 | SDR9C8 | Sterol/retinol dehydrogenase
- Origin Species
- Human
- SwissProt
- O75452
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLYLAVFVG LYYLLHWYRE RQVLSHLRDK YVFITGCDSG FGKLLARQLD ARGLRVLAAC61LTEKGAEQLR GQTSDRLETV TLDVTKTESV AAAAQWVKEC VRDKGLWGLV NNAGISLPTA121PNELLTKQDL LTILDVNLLG VIDVTLNLLP LVRRARGRVV NVFSVMGRVS LFGGGYCISK181YGVEAFSDSL RRELSYFGVK VAMIEPGYFK TAVTSKERFL KSFLEIWDRS SPEVKEAYGE241KFVADYKKSA EQMEQKCTQD LSLVTNCMEH ALIACHPRTR YSAGWDAKLL YLPMSYMPTF301LVDAIMYWVS PSPAKALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
317 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
