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Human RBM23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RBM23 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RBM23 antibodies →
$220.00each
Catalog Number: LS-G90807-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RBM23 (NM_001077351) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAPERbeta | RNA-binding motif protein 23 | Splicing factor SF2 | RNA binding motif protein 23 | RNPC4 | PP239 | RBM23
Origin Species
Hu
NCBI GenBank
NM_018107
SwissProt
Q86U06

Protein

Expression System
HEK293T
Fusion Tag
FLAG, Myc
Molecular Weight
48.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASDDFDIVI EAMLEAPYKK EEDEQQRKEV KKDYPSNTTS STSNSGNETS GSSTIGETSK
61KKRSRSHNKS RDRKRSRSRD RDRYRRRNSR SRSPGRQCRH RSRSWDRRHG SESRSRDHRR
121EDRVHYRSPP LATGYRYGHS KSPHFREKSP VREPVDNLSP EERDARTVFC MQLAARIRPR
181DLEDFFSAVG KVRDVRIISD RNSRRSKGIA YVEFCEIQSV PLAIGLTGQR LLGVPIIVQA
241SQAEKNRLAA MANNLQKGNG GPMRLYVGSL HFNITEDMLR GIFEPFGKID NIVLMKDSDT
301GRSKGYGFIT FSDSECARRA LEQLNGFELA GRPMRVGHVT ERLDGGTDIT FPDGDQELDL
361GSAGGRFQLM AKLAEGAGIQ LPSTAAAAAA AAAQAAALQL NGAVPLGALN PAALTALSPA
421LNLASQCFQL SSLFTPQTM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

439 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.