RAPGEFL1 Antibody
Catalog Number: LS-C126210-100
100 µg / $694.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RAPGEFL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G86399-20
Catalog Number: LS-G86399
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RAPGEFL1 (NM_016339) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Link-GEFII | RAPGEFL1 | Link GEFII
- Origin Species
- Human
- NCBI GenBank
- NM_016339
- SwissProt
- Q9UHV5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGPEGLGRK QACLAMLLHF LDTYQGLLQE EEGAGHIIKD LYLLIMKDES LYQGLREDTL61RLHQLVETVE LKIPEENQPP SKQVKPLFRH FRRIDSCLQT RVAFRGSDEI FCRVYMPDHS121YVTIRSRLSA SVQDILGSVT EKLQYSEEPA GREDSLILVA VSSSGEKVLL QPTEDCVFTA181LGINSHLFAC TRDSYEALVP LPEEIQVSPG DTEIHRVEPE DVANHLTAFH WELFRCVHEL241EFVDYVFHGE RGRRETANLE LLLQRCSEVT HWVATEVLLC EAPGKRAQLL KKFIKIAALC301KQNQDLLSFY AVVMGLDNAA VSRLRLTWEK LPGKFKNLFR KFENLTDPCR NHKSYREVIS361KMKPPVIPFV PLILKDLTFL HEGSKTLVDG LVNIEKLHSV AEKVRTIRKY RSRPLCLDME421ASPNHLQTKA YVRQFQVIDN QNLLFELSYK LEANSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
