RAPGEF6 Antibody (aa86-135)
Catalog Number: LS-C152109-100
100 µL (0.5 mg/mL) / $433.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RAPGEF6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89000-20
Catalog Number: LS-G89000
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RAGEF2(RAPGEF6) (NM_016340) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PDZGEF2 | KIA001LB | RA-GEF-2 | RAPGEF6 | PDZ-GEF2 | RAGEF2
- Origin Species
- Human
- NCBI GenBank
- NM_016340
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 179.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNSPVDPGAR QALRKKPPER TPEDLNTIYS YLHGMEILSN LREHQLRLMS ARARYERYSG61NQVLFCSETI ARCWYILLSG SVLVKGSMVL PPCSFGKQFG GKRGCDCLVL EPSEMIVVEN121AKDNEDSILQ REIPARQSRR RFRKINYKGE RQTITDDVEV NSYLSLPADL TKMHLTENPH181PQVTHVSSSQ SGCSIASDSG SSSLSDIYQA TESEVGDVDL TRLPEGPVDS EDDEEEDEEI241DRTDPLQGRD LVRECLEKEP ADKTDDDIEQ LLEFMHQLPA FANMTMSVRR ELCSVMIFEV301VEQAGAIILE DGQELDSWYV ILNGTVEISH PDGKVENLFM GNSFGITPTL DKQYMHGIVR361TKVDDCQFVC IAQQDYWRIL NHVEKNTHKV EEEGEIVMVH EHRELDRSGT RKGHIVIKAT421PERLIMHLIE EHSIVDPTYI EDFLLTYRTF LESPLDVGIK LLEWFKIDSL RDKVTRIVLL481WVNNHFNDFE GDPAMTRFLE EFEKNLEDTK MNGHLRLLNI ACAAKAKWRQ VVLQKASRES541PLQFSLNGGS EKGFGIFVEG VEPGSKAADS GLKRGDQIME VNGQNFENIT FMKAVEILRN601NTHLALTVKT NIFVFKELLF RTEQEKSGVP HIPKIAEKKS NRHSIQHVPG DIEQTSQEKG661SKKVKANTVS GGRNKIRKIL DKTRFSILPP KLFSDGGLSQ SQDDSIVGTR HCRHSLAIMP721IPGTLSSSSP DLLQPTTSML DFSNPSDIPD QVIRVFKVDQ QSCYIIISKD TTAKEVVFHA781VHEFGLTGAS DTYSLCEVSV TPEGVIKQRR LPDQFSKLAD RIQLNGRYYL KNNMETETLC841SDEDAQELVK ESQLSMLQLS TIEVATQLSM RDFDLFRNIE PTEYIDDLFK LNSKTGNTHL901KRFEDIVNQE TFWVASEILT EANQLKRMKI IKHFIKIALH CRECKNFNSM FAIISGLNLA961SVARLRGTWE KLPSKYEKHL QDLQDIFDPS RNMAKYRNIL SSQSMQPPII PLFPVVKKDM1021TFLHEGNDSK VDGLVNFEKL RMISKEIRQV VRMTSANMDP AMMFRQRSLS QGSTNSNMLD1081VQGGAHKKRA RRSSLLNAKK LYEDAQMARK VKQYLSSLDV ETDEEKFQMM SLQWEPAYGT1141LTKNLSEKRS AKSSEMSPVP MRSAGQTTKA HLHQPHRVSQ VLQVPAVNLH PIRKKGQTKD1201PALNTSLPQK VLGTTEEISG KKHTEDTISV ASSLHSSPPA SPQGSPHKGY TLIPSAKSDN1261LSDSSHSEIS SRSSIVSNCS VDSMSAALQD ERCSSQALAV PESTGALEKT EHASGIGDHS1321QHGPGWTLLK PSLIKCLAVS SSVSNEEISQ EHIIIEAADS GRGSWTSCSS SSHDNFQSLP1381NPKSWDFLNS YRHTHLDDPI AEVEPTDSEP YSCSKSCSRT CGQCKGSLER KSWTSSSSLS1441DTYEPNYGTV KQRVLESTPA ESSEGLDPKD ATDPVYKTVT SSTEKGLIVY CVTSPKKDDR1501YREPPPTPPG YLGISLADLK EGPHTHLKPP DYSVAVQRSK MMHNSLSRLP PASLSSNLVA1561CVPSKIVTQP QRHNLQPFHP KLGDVTDADS EADENEQVSA VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1601 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
