RALGPS1 Antibody
Catalog Number: LS-C749808
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RALGPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79754-20
Catalog Number: LS-G79754
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RALGPS1 (NM_001190728) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0351 | RalGEF 2 | RALGPS1A | RalA exchange factor RalGPS1 | RALGEF2 | RP13-225O21.1 | RALGPS1
- Origin Species
- Human
- NCBI GenBank
- AL929197
- SwissProt
- A2AR50
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL61MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE121ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK181EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII241ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE301DLAGPSAGSG SARFSRRPTC PDTSVAGSLP TPPVPRHRKS HSLGNNRGRL YATLGPNWRV361PVRNSPRTRS CVYSPTGPCI CSLGNSAAVP TMEGPLRRKT LLKEGRKPAL SSWTRYWVIL421SGSTLLYYGA KSLRGTDRKH VSIVGWMVQL PDDPEHPDIF QLNNPDKGNV YKFQTGSRFH481AILWHKHLDD ACKSNRPQEA GAAPGPTGTD SHEVDHLEGG AGKEAGPCATRTRPLEQKLISEEDLAANDILDYKDDDDKV
529 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
