PTCH2 / Patched 2 Antibody
Catalog Number: LS-C673600
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PTCH2 / Patched 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89197-20
Catalog Number: LS-G89197
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PTCH2 (NM_003738) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Patched (Drosophila) homolog 2 | Patched 2 | Patched homolog 2 | PTCH2 | Patched homolog 2 (Drosophila) | Protein patched homolog 2 | PTC2
- Origin Species
- Human
- NCBI GenBank
- NM_003738
- SwissProt
- Q9Y6C5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRSPPLREL PPSYTPPART AAPQILAGSL KAPLWLRAYF QGLLFSLGCG IQRHCGKVLF61LGLLAFGALA LGLRMAIIET NLEQLWVEVG SRVSQELHYT KEKLGEEAAY TSQMLIQTAR121QEGENILTPE ALGLHLQAAL TASKVQVSLY GKSWDLNKIC YKSGVPLIEN GMIERMIEKL181FPCVILTPLD CFWEGAKLQG GSAYLPGRPD IQWTNLDPEQ LLEELGPFAS LEGFRELLDK241AQVGQAYVGR PCLHPDDLHC PPSAPNHHSR QAPNVAHELS GGCHGFSHKF MHWQEELLLG301GMARDPQGEL LRAEALQSTF LLMSPRQLYE HFRGDYQTHD IGWSEEQAST VLQAWQRRFV361QLAQEALPEN ASQQIHAFSS TTLDDILHAF SEVSAARVVG GYLLMLAYAC VTMLRWDCAQ421SQGSVGLAGV LLVALAVASG LGLCALLGIT FNAATTQVLP FLALGIGVDD VFLLAHAFTE481ALPGTPLQER MGECLQRTGT SVVLTSINNM AAFLMAALVP IPALRAFSLQ AAIVVGCTFV541AVMLVFPAIL SLDLRRRHCQ RLDVLCCFSS PCSAQVIQIL PQELGDGTVP VGIAHLTATV601QAFTHCEASS QHVVTILPPQ AHLVPPPSDP LGSELFSPGG STRDLLGQEE ETRQKAACKS661LPCARWNLAH FARYQFAPLL LQSHAKAIVL VLFGALLGLS LYGATLVQDG LALTDVVPRG721TKEHAFLSAQ LRYFSLYEVA LVTQGGFDYA HSQRALFDLH QRFSSLKAVL PPPATQAPRT781WLHYYRNWLQ GIQAAFDQDW ASGRITRHSY RNGSEDGALA YKLLIQTGDA QEPLDFSQLT841TRKLVDREGL IPPELFYMGL TVWVSSDPLG LAASQANFYP PPPEWLHDKY DTTGENLRIP901PAQPLEFAQF PFLLRGLQKT ADFVEAIEGA RAACAEAGQA GVHAYPSGSP FLFWEQYLGL961RRCFLLAVCI LLVCTFLVCA LLLLNPWTAG LIVLVLAMMT VELFGIMGFL GIKLSAIPVV1021ILVASVGIGV EFTVHVALGF LTTQGSRNLR AAHALEHTFA PVTDGAISTL LGLLMLAGSH1081FDFIVRYFFA ALTVLTLLGL LHGLVLLPVL LSILGPPPEV IQMYKESPEI LSPPAPQGGG1141LRWGASSSLP QSFARVTTSM TVAIHPPPLP GAYIHPAPDE PPWSPAATSS GNLSSRGPGP1201ATGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1203 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
