Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRR5-ARHGAP8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G96125-20
Catalog Number: LS-G96125
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRR5 (NM_181334) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_181334
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPSLSDLG KREPAAAADE RGTQQRRACA NATWNSIHNG VIAVFQRKGL PDQELFSLNE61GVRQLLKTEL GSFFTEYLQN QLLTKGMVIL RDKIRFYEEL QRDKAAAAAV LGAVRKRPSV121VPMAGQDPAL STSHPFYDVA RHGILQVAGD DRFGRRVVTF SCCRMPPSHE LDHQRLLEYL181KYTLDQYVEN DYTIVYFHYG LNSRNKPSLG WLQSAYKEFD RKYKKNLKAL YVVHPTSFIK241VLWNILKPLI SHKFGKKVIY FNYLSELHEH LKYDQLVIPP EVLRYDEKLQ SLHEGRTPPP301TKTPPPRPPL PTQQFGVSLQ YLKDKNQGEL IPPVLRFTVT YLREKGLRTE GLFRRSASVQ361TVREIQRLYN QGKPVNFDDY GDIHIPAVIL KTFLRELPQP LLTFQAYEQI LGITCVESSL421RVTGCRQILR SLPEHNYVVL RYLMGFLHAV SRESIFNKMN SSNLACVFGL NLIWPSQGVS481SLSALVPLNM FTELLIEYYE KIFSTPEAPG EHGLAPWEQG SRAAPLQEAV PRTQATGLTK541PTLPPSPLMA ARRRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.