Human PRR14L Protein (Recombinant His, N-Terminal)
Catalog Number: LS-G144785-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRR14L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92136-20
Catalog Number: LS-G92136
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C22ORF30(PRR14L) (NM_173566) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Proline rich 14-like protein | Proline rich 14-like | PRR14L | C22orf30 | Protein PRR14L | RP4-694E4.2
- Origin Species
- Human
- NCBI GenBank
- AK026712
- SwissProt
- Q5THK1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQRLFMTQFT QGLKGLRSPA SIADKVFCSL PYSVGRVLSI WSQHGPSVCS FEISSLHSPH61CKRQPSLGTT SSHTMLPYVP LPGMEATYNT SGSQTRLEPP FPALVPKSCL VAESAVSKLL121LSASEFQVRG LDELDGVKAA CPCPQSSPPE QKEAEPEKRP KKVSQIRIRK TIPRPDPNLT181PMGLPRPKRL KKKEFSLEEI YTNKNYKSPP ANRDLDSSCP STDSETGHFL VFGYAQRQAQ241PHPLLASRRL IGCSSPEGRG HPRSYPYWRR LLVLCWYLPG WRAGRVTWLR LATWAGRGDS301SLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
302 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
