PRKCZ / PKC-Zeta Antibody
Catalog Number: LS-C500310
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRKCZ / PKC-Zeta Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78331-20
Catalog Number: LS-G78331
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PKC zeta(PRKCZ) (NM_001033582) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PKC-ZETA | PKC2 | Pkcz | PKCzeta | Protein kinase c zeta | Protein kinase C zeta type | NPKC-zeta | PKC Zeta | PRKCZ | Protein kinase C | zeta | Protein kinase Czeta
- Origin Species
- Human
- NCBI GenBank
- NM_002744
- SwissProt
- Q05513
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP VIDGMDGIKI61SQGLGLQDFD LIRVIGRGSY AKVLLVRLKK NDQIYAMKVV KKELVHDDED IDWVQTEKHV121FEQASSNPFL VGLHSCFQTT SRLFLVIEYV NGGDLMFHMQ RQRKLPEEHA RFYAAEICIA181LNFLHERGII YRDLKLDNVL LDADGHIKLT DYGMCKEGLG PGDTTSTFCG TPNYIAPEIL241RGEEYGFSVD WWALGVLMFE MMAGRSPFDI ITDNPDMNTE DYLFQVILEK PIRIPRFLSV301KASHVLKGFL NKDPKERLGC RPQTGFSDIK SHAFFRSIDW DLLEKKQALP PFQPQITDDY361GLDNFDTQFT SEPVQLTPDD EDAIKRIDQS EFEGFEYINP LLLSTEESVSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
409 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
