LSBio
Human PPP2R2C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PPP2R2C Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PPP2R2C antibodies →
$220.00each
Catalog Number: LS-G87642-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R2C (NM_181876) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B55-GAMMA | IMYPNO | IMYPNO1 | PP2A subunit B isoform gamma | PPP2R2C | PR52 | PR55G
Origin Species
Human
NCBI GenBank
AF086924
SwissProt
Q9Y2T4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MGLSFFSKHL PIQEGQPWAL KTPADIISTV EFNHTGELLA TGDKGGRVVI FQREPESKNA
61PHSQGEYDVY STFQSHEPEF DYLKSLEIEE KINKIKWLPQ QNAAHSLLST NDKTIKLWKI
121TERDKRPEGY NLKDEEGKLK DLSTVTSLQV PVLKPMDLMV EVSPRRIFAN GHTYHINSIS
181VNSDCETYMS ADDLRINLWH LAITDRSFNI VDIKPANMED LTEVITASEF HPHHCNLFVY
241SSSKGSLRLC DMRAAALCDK HSKLFEEPED PSNRSFFSEI ISSVSDVKFS HSGRYMLTRD
301YLTVKVWDLN MEARPIETYQ VHDYLRSKLC SLYENDCIFD KFECAWNGSD SVIMTGAYNN
361FFRMFDRNTK RDVTLEASRE SSKPRAVLKP RRVCVGGKRR RDDISVDSLD FTKKILHTAW
421HPAENIIAIA ATNNLYIFQD KVNSDMH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

447 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.