PPEF / PPEF1 Antibody
Catalog Number: LS-C155387-100
100 µL (1 mg/mL) / $459.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPEF / PPEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G80333-20
Catalog Number: LS-G80333
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPEF1 (NM_152224) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PPEF | PPP7C | PP7 | PPEF-1 | PPEF1 | PPP7CA | Protein phosphatase PP7
- Origin Species
- Human
- NCBI GenBank
- NM_006240
- SwissProt
- O14829
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGCSSSSTKT RRSDTSLRAA LIIQNWYRGY KARLKARQHY ALTIFQSIEY ADEQGQMQLS61TFFSFMLENY THIHKEELEL RNQSLESEQD MRDRWDYVDS IDVPDSYNGP RLQFPLTCTD121IDLLLEAFKE QQILHAHYVL EVLFETKKVL KQMPNFTHIQ TSPSKEVTIC GDLHGKLDDL181FLIFYKNGLP SERNPYVFNG DFVDRGKNSI EILMILCVSF LVYPNDLHLN RGNHEDFMMN241LRYGFTKEIL HKYKLHGKRI LQILEEFYAW LPIGTIVDNE ILVIHGGISE TTDLNLLHRV301ERNKMKSVLI PPTETNRDHD TDSKHNKIID ILWSDPRGKN GCFPNTCRGG GCYFGPDVTS361KILNKYQLKM LIRSHECKPE GYEICHDGKV VTIFSASNYY EEGSNRGAYI KLCSGTTPRF421FQYQVTKATC FQPLRQRVDT MENSAIKILR ERVISRKSDL TRAFQLQDHR KSGKLSVSQW481AFCMENILGL NLPWRSLSSN LVNIDQNGNV EYMSSFQNIR IEKPVQEAHS TLVETLYRYR541SDLEIIFNAI DTDHSGLISV EEFRAMWKLF SSHYNVHIDD SQVNKLANIM DLNKDGSIDF601NEFLKAFYVV HRYEDLMKPD VTNLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
