
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPAPDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PLPP6 (NM_203453) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA6J24.6 | PPAPDC2 | PSDP
- Origin Species
- Human
- NCBI GenBank
- NM_203453
- SwissProt
- Q8IY26
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSPRRSMEG RPLGVSASSS SSSPGSPAHG GGGGGSRFEF QSLLSSRATA VDPTCARLRA61SESPVHRRGS FPLAAAGPSQ SPAPPLPEED RMDLNPSFLG IALRSLLAID LWLSKKLGVC121AGESSSWGSV RPLMKLLEIW GHGIPWLLGT LYCLCRSDSW AGREVLMNLL FALLLDLLLV181ALIKGLVRRR RPAHNQMDMF VTLSVDKYSF PSGHATRAAL MSRFILNHLV LAIPLRVLVV241LWAFVLGLSR VMLGRHNVTD VAFGFFLGYM QYSIVDYCWL SPHNAPVLFL LWSQRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
295 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.













