LSBio
Human POMGNT2 / GTDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human POMGNT2 / GTDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all POMGNT2 antibodies →
$153.00each
Catalog Number: LS-G87795-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C3orf39(POMGNT2) (NM_032806) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGO61 | EOGTL | MDDGA8 | GTDC2 | C3orf39
Origin Species
Human
NCBI GenBank
NM_032806
SwissProt
Q8NAT1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHLSAVFNAL LVSVLAAVLW KHVRLREHAA TLEEELALSR QATEPAPALR IDYPKALQIL
61MEGGTHMVCT GRTHTDRICR FKWLCYSNEA EEFIFFHGNT SVMLPNLGSR RFQPALLDLS
121TVEDHNTQYF NFVELPAAAL RFMPKPVFVP DVALIANRFN PDNLMHVFHD DLLPLFYTLR
181QFPGLAHEAR LFFMEGWGEG AHFDLYKLLS PKQPLLRAQL KTLGRLLCFS HAFVGLSKIT
241TWYQYGFVQP QGPKANILVS GNEIRQFARF MTEKLNVSHT GVPLGEEYIL VFSRTQNRLI
301LNEAELLLAL AQEFQMKTVT VSLEDHTFAD VVRLVSNASM LVSMHGAQLV TTLFLPRGAT
361VVELFPYAVN PDHYTPYKTL AMLPGMDLQY VAWRNMMPEN TVTHPERPWD QGGITHLDRA
421EQARILQSRE VPRHLCCRNP EWLFRIYQDT KVDIPSLIQT IRRVVKGRPG PRKQKWTVGL
481YPGKVREARC QASVHGASEA RLTVSWQIPW NLKYLKVREV KYEVWLQEQG ENTYVPYILA
541LQNHTFTENI KPFTTYLVWV RCIFNKILLG PFADVLVCNT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

580 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.