PNPLA1 Antibody (HRP)
Catalog Number: LS-C397580
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PNPLA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G93996-20
Catalog Number: LS-G93996
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PNPLA1 (NM_173676) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ50J22.1 | PNPLA1
- Origin Species
- Human
- SwissProt
- Q8N8W4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVQMMRQFLY RVLPEDSYKV TTGKLHVSLT RLTDGENVVV SEFTSKEELI EALYCSCFVP61VYCGLIPPTY RGVRYIDGGF TGMQPCAFWT DAITISTFSG QQDICPRDCP AIFHDFRMFN121CSFQFSLENI ARMTHALFPP DLVILHDYYY RGYEDAVLYL RRLNAVYLNS SSKRVIFPRV181EVYCQIELAL GNECPERSQP SLRARQASLE GATQPHKEWV PKGDGRGSHG PPVSQPVQTL241EFTCESPVSA PVSPLEQPPA QPLASSTPLS LSGMPPVSFP AVHKPPSSTP GSSLPTPPPG301LSPLSPQQQV QPSGSPARSL HSQAPTSPRP SLGPSTVGAP QTLPRSSLSA FPAQPPVEEL361GQEQPQAVAL LVSSKPKSAV PLVHVKETVS KPYVTESPAE DSNWVNKVFK KNKQKTSGTR421KGFPRHPGSK KPSSKVQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
437 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
