LSBio
Human PLS3 / T Plastin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PLS3 / T Plastin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G79093-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human T Plastin(PLS3) (NM_001172335) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
T plastin | T-plastin | T fimbrin | Plastin 3 | Plastin-3 | PLS3 | Plastin 3 (T isoform)
Origin Species
Human
NCBI GenBank
NM_005032
SwissProt
P13797

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MDEMATTQIS KDELDELKEA FAKVDLNSNG FICDYELHEL FKEANMPLPG YKIFQEVKSS
61DIAKTFRKAI NRKEGICALG GTSELSSEGT QHSYSEEEKY AFVNWINKAL ENDPDCRHVI
121PMNPNTDDLF KAVGDGIVLC KMINLSVPDT IDERAINKKK LTPFIIQENL NLALNSASAI
181GCHVVNIGAE DLRAGKPHLV LGLLWQIIKI GLFADIELSR NEALAALLRD GETLEELMKL
241SPEELLLRWA NFHLENSGWQ KINNFSADIK DSKAYFHLLN QIAPKGQKEG EPRIDINMSG
301FNETDDLKRA ESMLQQADKL GCRQFVTPAD VVSGNPKLNL AFVANLFNKY PALTKPENQD
361IDWTLLEGET REERTFRNWM NSLGVNPHVN HLYADLQDAL VILQLYERIK VPVDWSKVNK
421PPYPKLGANM KKLENCNYAV ELGKHPAKFS LVGIGGQDLN DGNQTLTLAL VWQLMRRYTL
481NVLEDLGDGQ KANDDIIVNW VNRTLSEAGK STSIQSFKDK TISSSLAVVD LIDAIQPGCI
541NYDLVKSGNL TEDDKHNNAK YAVSMARRIG ARVYALPEDL VEVKPKMVMT VFACLMGRGM
601KRV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.