PLB1 Antibody
Catalog Number: LS-C668990
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PLB1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95869-20
Catalog Number: LS-G95869
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Phospholipase A2(PLB1) (NM_153021) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PLB | Phospholipase B/lipase | Phospholipase B | PLB/LIP
- Origin Species
- Human
- NCBI GenBank
- NM_153021
- SwissProt
- Q6P1J6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 162.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLRPGIFLL ELLLLLGQGT PQIHTSPRKS TLEGQLWPET LKNSPFPCNP NKLGVNMPSK61SVHSLKPSDI KFVAAIGNLE IPPDPGTGDL EKQDWTERPQ QVCMGVMTVL SDIIRYFSPS121VPMPVCHTGK RVIPHDGAED LWIQAQELVR NMKENLQLDF QFDWKLINVF FSNASQCYLC181PSAQQNGLAA GGVDELMGVL DYLQQEVPRA FVNLVDLSEV AEVSRQYHGT WLSPAPEPCN241CSEETTRLAK VVMQWSYQEA WNSLLASSRY SEQESFTVVF QPFFYETTPS LHSEDPRLQD301STTLAWHLWN RMMEPAGEKD EPLSVKHGRP MKCPSQESPY LFSYRNSNYL TRLQKPQDKL361EVREGAEIRC PDKDPSDTVP TSVHRLKPAD INVIGALGDS LTAGNGAGST PGNVLDVLTQ421YRGLSWSVGG DENIGTVTTL ANILREFNPS LKGFSVGTGK ETSPNAFLNQ AVAGGRAEDL481PVQARRLVDL MKNDTRIHFQ EDWKIITLFI GGNDLCDFCN DLVHYSPQNF TDNIGKALDI541LHAEVPRAFV NLVTVLEIVN LRELYQEKKV YCPRMILRSL CPCVLKFDDN STELATLIEF601NKKFQEKTHQ LIESGRYDTR EDFTVVVQPF FENVDMPKTS EGLPDNSFFA PDCFHFSSKS661HSRAASALWN NMLEPVGQKT TRHKFENKIN ITCPNQVQPF LRTYKNSMQG HGTWLPCRDR721APSALHPTSV HALRPADIQV VAALGDSLTA GNGIGSKPDD LPDVTTQYRG LSYSAGGDGS781LENVTTLPNI LREFNRNLTG YAVGTGDAND TNAFLNQAVP GAKAEDLMSQ VQTLMQKMKD841DHRVNFHEDW KVITVLIGGS DLCDYCTDSN LYSAANFVDH LRNALDVLHR EVPRVLVNLV901DFLNPTIMRQ VFLGNPDKCP VQQASVLCNC VLTLRENSQE LARLEAFSRA YRSSMRELVG961SGRYDTQEDF SVVLQPFFQN IQLPVLADGL PDTSFFAPDC IHPNQKFHSQ LARALWTNML1021EPLGSKTETL DLRAEMPITC PTQNEPFLRT PRNSNYTYPI KPAIENWGSD FLCTEWKASN1081SVPTSVHQLR PADIKVVAAL GDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT1141LPNILKKFNP YLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKD1201WKLVTLFIGV NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNV VEVMELASLY1261QGQGGKCAML AAQNNCTCLR HSQSSLEKQE LKKVNWNLQH GISSFSYWHQ YTQREDFAVV1321VQPFFQNTLT PLNERGDTDL TFFSEDCFHF SDRGHAEMAI ALWNNMLEPV GRKTTSNNFT1381HSRAKLKCPS PESPYLYTLR NSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC1441RRGGRREDPP MSLRTVALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1458 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
