LSBio
Human PKP2 / Plakophilin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PKP2 / Plakophilin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PKP2 antibodies →
$153.00each
Catalog Number: LS-G85970-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Plakophilin 2(PKP2) (NM_001005242) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARVD9 | PKP2 | Plakophilin 2 | Plakophilin-2
Origin Species
Human
NCBI GenBank
NM_004572
SwissProt
Q99959

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPGAPAEY GYIRTVLGQQ ILGQLDSSSL ALPSEAKLKL AGSSGRGGQT VKSLRIQEQV
61QQTLARKGRS SVGNGNLHRT SSVPEYVYNL HLVENDFVGG RSPVPKTYDM LKAGTTATYE
121GRWGRGTAQY SSQKSVEERS LRHPLRRLEI SPDSSPERAH YTHSDYQYSQ RSQAGHTLHH
181QESRRAALLV PPRYARSEIV GVSRAGTTSR QRHFDTYHRQ YQHGSVSDTV FDSIPANPAL
241LTYPRPGTSR SMGNLLEKEN YLTAGLTVGQ VRPLVPLQPV TQNRASRSSW HQSSFHSTRT
301LREAGPSVAV DSSGRRAHLT VGQAAAGGSG NLLTERSTFT DSQLGNADME MTLERAVSML
361EADHMPPSRI SAAATFIQHE CFQKSEARKR VNQLRGILKL LQLLKVQNED VQRAVCGALR
421NLVFEDNDNK LEVAELNGVP RLLQVLKQTR DLETKKQITG LLWNLSSNDK LKNLMITEAL
481LTLTENIIIP FSGWPEGDYP KANGLLDFDI FYNVTGCLRN MSSAGADGRK AMRRCDGLID
541SLVHYVRGTI ADYQPDDKAT ENCVCILHNL SYQLEAELPE KYSQNIYIQN RNIQTDNNKS
601IGCFGSRSRK VKEQYQDVPM PEEKSNPKGV EWLWHSIVIR MYLSLIAKSV RNYTQEASLG
661ALQNLTAGSG PMPTSVAQTV VQKESGLQHT RKMLHVGDPS VKKTAISLLR NLSRNLSLQN
721EIAKETLPDL VSIIPDTVPS TDLLIETTAS ACYTLNNIIQ NSYQNARDLL NTGGIQKIMA
781ISAGDAYASN KASKAASVLL YSLWAHTELH HAYKKAQFKK TDFVNSRTAK AYHSLKD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

837 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.