PIGT Antibody
Catalog Number: LS-C747301
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PIGT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G84094-20
Catalog Number: LS-G84094
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PIGT (NM_001184728) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CGI-06 | GPI transamidase subunit | NDAP | PIGT
- Origin Species
- Human
- NCBI GenBank
- NM_015937
- SwissProt
- Q969N2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAMPLALL VLLLLGPGGW CLAEPPRDSL REELVITPLP SGDVAATFQF RTRWDSELQR61EGVSHYRLFP KALGQLISKY SLRELHLSFT QGFWRTRYWG PPFLQAPSDT DHYFLRYAVL121PREVVCTENL TPWKKLLPCS SKAGLSVLLK ADRLFHTSYH SQAVHIRPVC RNARCTSISW181ELRQTLSVVF DAFITGQGKK DWSLFRMFSR TLTEPCPLAS ESRVYVDITT YNQDNETLEV241HPPPTTTYQD VILGTRKTYA IYDLLDTAMI NNSRNLNIQL KWKRPPENEA PPVPFLHAQR301YVSGYGLQKG ELSTLLYNTH PYRAFPVLLL DTVPWYLRLY VHTLTITSKG KENKPSYIHY361QPAQDRLQPH LLEMLIQLPA NSVTKVSIQF ERALLKWTEY TPDPNHGFYV SPSVLSALVP421SMVAAKPVDW EESPLFNSLF PVSDGSNYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV481AVCYGSFYNL LTRTFHIEEP RTGGLAKRLA NLIRRARGVP PLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
522 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
