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Human PHTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PHTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PHTF1 antibodies →
$220.00each
Catalog Number: LS-G89527-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PHTF1 (NM_006608) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHTF | PHTF1
Origin Species
Human
NCBI GenBank
NM_006608
SwissProt
Q9UMS5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASNERDAIS WYQKKIGAYD QQIWEKSIEQ TQIKGLKNKP KKMGHIKPDL IDVDLIRGST
61FAKAKPEIPW TSLTRKGLVR VVFFPLFSNW WIQVTSLRIF VWLLLLYFMQ VIAIVLYLMM
121PIVNISEVLG PLCLMLLMGT VHCQIVSTQI TRPSGNNGNR RRRKLRKTVN GDGSRENGNN
181SSDKVRGIET LESVPIIGGF WETIFGNRIK RVKLISNKGT ETDNDPSCVH PIIKRRQCRP
241EIRMWQTREK AKFSDGEKCR REAFRRLGNG VSDDLSSEED GEARTQMILL RRSVEGASSD
301NGCEVKNRKS ILSRHLNSQV KKTTTRWCHI VRDSDSLAES EFESAAFSQG SRSGVSGGSR
361SLNMSRRDSE STRHDSETED MLWDDLLHGP ECRSSVTSDS EGAHVNTLHS GTKRDPKEDV
421FQQNHLFWLQ NSSPSSDRVS AIIWEGNECK KMDMSVLEIS GIIMSRVNAY QQGVGYQMLG
481NVVTIGLAFF PFLHRLFREK SLDQLKSISA EEILTLFCGA PPVTPIIVLS IINFFERLCL
541TWMFFFMMCV AERTYKQRFL FAKLFSHITS ARKARKYEIP HFRLKKVENI KIWLSLRSYL
601KRRGPQRSVD VVVSSVFLLT LSIAFICCAQ VLQGHKTFLN DAYNWEFLIW ETALLLFLLR
661LASLGSETNK KYSNVSILLT EQINLYLKME KKPNKKEQLT LVNNVLKLST KLLKELDTPF
721RLYGLTMNPL IYNITRVVIL SAVSGVISDL LGFNIRLWKI KS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

762 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.