LSBio
Human PGM1 / Phosphoglucomutase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PGM1 / Phosphoglucomutase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PGM1 antibodies →
$153.00each
Catalog Number: LS-G78014-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PGM1 (NM_001172818) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDG1T | Glucose phosphomutase 1 | GSD14 | PGM1 | Phosphoglucomutase 1 | PGM 1 | Phosphoglucomutase-1 | Phosphoglucomutase
Origin Species
Human
NCBI GenBank
NM_002633
SwissProt
P36871

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDFEEWISG TYRKMEEGPL PLLTFATAPY HDQKPGTSGL RKKTYYFEEK PCYLENFIQS
61IFFSIDLKDR QGSSLVVGGD GRYFNKSAIE TIVQMAAANG IGRLVIGQNG ILSTPAVSCI
121IRKIKAIGGI ILTASHNPGG PNGDFGIKFN ISNGGPAPEA ITDKIFQISK TIEEYAVCPD
181LKVDLGVLGK QQFDLENKFK PFTVEIVDSV EAYATMLRSI FDFSALKELL SGPNRLKIRI
241DAMHGVVGPY VKKILCEELG APANSAVNCV PLEDFGGHHP DPNLTYAADL VETMKSGEHD
301FGAAFDGDGD RNMILGKHGF FVNPSDSVAV IAANIFSIPY FQQTGVRGFA RSMPTSGALD
361RVASATKIAL YETPTGWKFF GNLMDASKLS LCGEESFGTG SDHIREKDGL WAVLAWLSIL
421ATRKQSVEDI LKDHWQKYGR NFFTRYDYEE VEAEGANKMM KDLEALMFDR SFVGKQFSAN
481DKVYTVEKAD NFEYSDPVDG SISRNQGLRL IFTDGSRIVF RLSGTGSAGA TIRLYIDSYE
541KDVAKINQDP QVMLAPLISI ALKVSQLQER TGRTAPTVIT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

580 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.