PGD Antibody
Catalog Number: LS-C667617-100
100 µg / $338.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Human PGD Protein (Recombinant His)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Liquid |
$260.00each
Catalog Number: LS-G728-20
Catalog Number: LS-G728
For bulk orders or custom formulations:
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- Description
- PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- 6PGD | PGDH | Phosphogluconate dehydrogenase | PGD
- Origin Species
- Human
- NCBI GenBank
- NM_002631|NP_002622.2
- SwissProt
- P52209
Protein
- Expression System
- Escherichia coli (Prokaryotic)
- Fusion Tag
- Hexahistidine Tag
- Protein Type
- Recombinant
- Amino Acid Sequence
11-483aa|MG SSHHHHHHSS GLVPRGSHMA QADIALIGLA VMGQNLILNM NDHGFVVCAF61NRTVSKVDDF LANEAKGTKV VGAQSLKEMV SKLKKPRRII LLVKAGQAVD DFIEKLVPLL121DTGDIIIDGG NSEYRDTTRR CRDLKAKGIL FVGSGVSGGE EGARYGPSLM PGGNKEAWPH181IKTIFQGIAA KVGTGEPCCD WVGDEGAGHF VKMVHNGIEY GDMQLICEAY HLMKDVLGMA241QDEMAQAFED WNKTELDSFL IEITANILKF QDTDGKHLLP KIRDSAGQKG TGKWTAISAL301EYGVPVTLIG EAVFARCLSS LKDERIQASK KLKGPQKFQF DGDKKSFLED IRKALYASKI361ISYAQGFMLL RQAATEFGWT LNYGGIALMW RGGCIIRSVF LGKIKDAFDR NPELQNLLLD421DFFKSAVENC QDSWRRAVST GVQAGIPMPC FTTALSFYDG YRHEMLPASL IQAQRDYFGA481HTYELLAKPG QFIHTNWTGH GGTVSSSSYN A
511 aa
Format
- Purity
- >95%
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM DTT, 10% Glycerol
- Purity Measurement Method
- SDS-PAGE (Coomassie Stain)
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
