PDE7A Antibody
Catalog Number: LS-C778393-100
100 µL / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PDE7A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81132-20
Catalog Number: LS-G81132
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PDE7A (NM_002603) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PDE7A1 | PDE7A | PDE7A2 | Phosphodiesterase 7A | Phosphodiesterase isozyme 7 | PDE7 | HCP1 | TM22
- Origin Species
- Human
- NCBI GenBank
- L12052
- SwissProt
- Q13946
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGITLIWCLA LVLIKWITSK RRGAISYDSS DQTALYIRML GDVRVRSRAG FESERRGSHP61YIDFRIFHSQ SEIEVSVSAR NIRRLLSFQR YLRSSRFFRG TAVSNSLNIL DDDYNGQAKC121MLEKVGNWNF DIFLFDRLTN GNSLVSLTFH LFSLHGLIEY FHLDMMKLRR FLVMIQEDYH181SQNPYHNAVH AADVTQAMHC YLKEPKLANS VTPWDILLSL IAAATHDLDH PGVNQPFLIK241TNHYLATLYK NTSVLENHHW RSAVGLLRES GLFSHLPLES RQQMETQIGA LILATDISRQ301NEYLSLFRSH LDRGDLCLED TRHRHLVLQM ALKCADICNP CRTWELSKQW SEKVTEEFFH361QGDIEKKYHL GVSPLCDRHT ESIANIQIGF MTYLVEPLFT EWARFSNTRL SQTMLGHVGL421NKASWKGLQR EQSSSEDTDA AFELNSQLLP QENRLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
