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Human PCNXL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PCNXL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PCNXL3 antibodies →
$220.00each
Catalog Number: LS-G96092-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PCNXL3(PCNX3) (NM_032223) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032223

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
221.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSQVLQILR QGVWASLTGG WFFDPHQSTF SNCFHLYVWI FLLIFPFLLY MVLPPSLMVA
61GVYCLVVAVI FATIKTVNYR LHAMFDQGEI VEKRSSTMGE LEEEPAQGDS NPPRDPGVEM
121TVFRKVSSTP PVRCSSQHSV FGFNQVSELL PRMEDSGPLR DIKELVREQG SNNVIVTSAD
181REMLKLSSQE KLIGDLPQTP PGAVPDPSLA STDSSEPSPL AGDGAPWSGS SMADTPMSPL
241LKGSLSQELS KSFLTLTQPD RALVRTSSRR EQRRGAGGYQ PLDRRGSGEP TPQKAGSSDS
301CFSGTDRETL SSFKSEKTNS THLDSPPGGP APEGSDTDPP SEAELPASPD AGVPSDDTLR
361SFDTVIGAGT PPGLAEPLLV VRPKDLALLR PSKRQPPLRR HSPPGRAPRR PLLEGGGFFE
421DEDTSEGSEL SPASSLRSQR RYSTDSSSST SCYSPESSRG AAGGPRKRRA PHGAEEGTAV
481PPKRPYGTQR TPSTASAKTH ARVLSMDGAG GDVLRPPLAG CKAELEAQVG VEQAASEPVV
541LPAEARRGPA ANQPGWRGEL QEEGAVGGAA EETGRRDRSS SVRRTQAIRR RHNAGSNPTP
601PASVMGSPPS SLQEAQRGRA ASHSRALTLP SALHFASSLL LTRAGANVHE ACTFDDTSEG
661AVHYFYDESG VRRSYTFGLA GGGYENPVGQ QGEQTANGAW DRHSHSSSFH SADVPEATGG
721LNLLQPRPVV LQGMQVRRVP LEIPEEQTLM EEAPPRAQHS YKYWLLPGRW TSVRYERLAL
781LALLDRTRGV LENIFGVGLS SLVAFLGYLL LLKGFFTDIW VFQFCLVIAS CQYSLLKSVQ
841PDAASPMHGH NWVIAYSRPV YFCICCLLIW LLDALGSAQP FPPVSLYGLT LFSASFFFCA
901RDVATVFTLC FPFVFLLGLL PQVNTCLMYL LEQIDMHGFG GTAATSPLTA VFSLSRSLLA
961AALLYGFCLG AIKTPWPEQH VPVLFSVFCG LLVALSYHLS RQSSDPTVLW SLIRSKLFPE
1021LEERSLETAR AEPPDPLPDK MRQSVREVLH SDLVMCVVIA VLTFAISAST VFIALKSVLG
1081FVLYALAGAV GFFTHYLLPQ LRKQLPWFCL SQPVLKPLEY SQYEVRGAAQ VMWFEKLYAG
1141LQCVEKYLIY PAVVLNALTV DAHTVVSHPD KYCFYCRALL MTVAGLKLLR SAFCCPPQQY
1201LTLAFTVLLF HFDYPRLSQG FLLDYFLMSL LCSKLWDLLY KLRFVLTYIA PWQITWGSAF
1261HAFAQPFAVP HSAMLFVQAL LSGLFSTPLN PLLGSAVFIM SYARPLKFWE RDYNTKRVDH
1321SNTRLVTQLD RNPGADDNNL NSIFYEHLTR SLQHTLCGDL VLGRWGNYGP GDCFVLASDY
1381LNALVHLIEV GNGLVTFQLR GLEFRGTYCQ QREVEAITEG VEEDEGCCCC EPGHLPRVLS
1441FNAAFGQRWL AWEVTASKYV LEGYSISDNN AASMLQVFDL RKILITYYVK SIIYYVSRSP
1501KLEVWLSHEG ITAALRPVRV PGYADSDPTF SLSVDEDYDL RLSGLSLPSF CAVHLEWIQY
1561CASRRSQPVD QDWNSPLVTL CFGLCVLGRR ALGTASHSMS ASLEPFLYGL HALFKGDFRI
1621TSPRDEWVFA DMDLLHRVVA PGVRMALKLH QDHFTSPDEY EEPAALYDAI AANEERLVIS
1681HEGDPAWRSA ILSNTPSLLA LRHVLDDASD EYKIIMLNRR HLSFRVIKVN RECVRGLWAG
1741QQQELVFLRN RNPERGSIQN AKQALRNMIN SSCDQPLGYP IYVSPLTTSL AGSHPQLRAL
1801WGGPISLGAI AHWLLRTWER LHKGCGAGCN SGGNVDDSDC SGGGGLTSLS NNPPVAHPTP
1861ENTAGNGDQP LPPGPGWGPR SSLSGSGDGR PPPLLQWPPP RLPGPPPASP IPTEGPRTSR
1921PPGPGLLSSE GPSGKWSLGG RKGLGGSDGE PASGSPKGGT PKSQAPLDLS LSLSLSLSPD
1981VSTEASPPRA SQDIPCLDSS APESGTPMGA LGDWPAPIEE RESPAAQPLL EHQY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2034 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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