LSBio
Human PCA1 / ALKBH3 Protein (Recombinant His)

Human PCA1 / ALKBH3 Protein (Recombinant His)

Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
See all ALKBH3 antibodies →
$267.00each
Catalog Number: LS-G1202-20

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Overview

Description
ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. Recombinant human ALKBH3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Gene Name
ALKBH3
Alternative Names
ALKBH3 | ABH3 | DEPC1 | DEPC-1 | Prostate cancer antigen 1 | PCA1 | Prostate cancer antigen-1
Origin Species
Human
UniProt Number
Q96Q83
SwissProt
Q96Q83

Protein

Expression System
Escherichia coli (Prokaryotic)
Fusion Tag
Hexahistidine Tag
Protein Type
Recombinant
Amino Acid Sequence
11-286aa|MR GSHHHHHHGM ASMTGGQQMG RDLYDDDDKD RWGSELDMEE KRRRARVQGA
61WAAPVKSQAI AQPATTAKSH LHQKPGQTWK NKEHHLSDRE FVFKEPQQVV RRAPEPRVID
121REGVYEISLS PTGVSRVCLY PGFVDVKEAD WILEQLCQDV PWKQRTGIRE DITYQQPRLT
181AWYGELPYTY SRITMEPNPH WHPVLRTLKN RIEENTGHTF NSLLCNLYRN EKDSVDWHSD
241DEPSLGRCPI IASLSFGATR TFEMRKKPPP EENGDYTYVE RVKIPLDHGT LLIMEGATQA
301DWQHRVPKEY HSREPRVNLT FRTVYPDPRG APW

333 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.

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